中国中西医结合杂志2024，Vol.44Issue(11)：1339-1345,7.DOI:10.7661/j.cjim.20240207.126
温肾通督方通过减轻铁过载促进脊髓损伤修复
Wenshen Tongdu Recipe Promotes Spinal Cord Injury Repairment by Reducing Iron Overload
摘要
Abstract
Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)in promoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the inflammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham-operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P＜0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-α in serum and spinal cord tissues significantly increased in the model group(P＜0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-α were significantly lowered in DFO group and WSTDR group(P＜0.05,P＜0.01).Serum concentrations of MCP-1 and IL-1β were significantly lowered in WSTDR+DFO group(P＜0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,and IL-1β were significantly improved in WSTDR+DFO group(P＜0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P＜0.05,P＜0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promoted MECs to engulf myelin debris by reducing iron overload,improving the inflammatory microenvironment,and promoting spinal cord injury repairment.关键词
脊髓损伤/温肾通督方/微血管内皮细胞/髓鞘碎片/铁过载/中药复方Key words
spinal cord injury/Wenshen Tongdu Recipe/microvascular endothelial cell/myelin debris/iron overload/Chinese herbal compound引用本文复制引用
陈思娴,马勇,石磊,吴承杰,赵瑞华,郭杨,潘娅岚,吴毛,刘孟敏..温肾通督方通过减轻铁过载促进脊髓损伤修复[J].中国中西医结合杂志,2024,44(11):1339-1345,7.基金项目
国家自然科学基金面上项目(No.81973885,No.82174400) （No.81973885,No.82174400）
南京中医药大学自然科学基金青年项目(No.NZY81704100) （No.NZY81704100）
江苏省高校自然科学基金项目(No.21KJB360006) （No.21KJB360006）
江苏省中医退行性骨关节病临床医学创新中心资助项目(No.苏中医科教[2021]4号) （No.苏中医科教[2021]4号）
2022江苏省研究生科研与实践创新计划项目(No.KYCX22_1942) （No.KYCX22_1942）
2023年江苏省研究生科研创新计划项目(No.KYCX23_2205,No.KYCX23_2207) （No.KYCX23_2205,No.KYCX23_2207）
