中国中西医结合杂志2024，Vol.44Issue(11)：1365-1371,7.DOI:10.7661/j.cjim.20240202.125
苦参通过抑制MAPK/NF-κB通路改善免疫平衡缓解特应性皮炎实验研究
Sophora flavescens Alleviated Atopic Dermatitis by Improving Immune Balance and Inhibiting MAPK/NF-κB Signaling Pathways
摘要
Abstract
Objective To study the therapeutic effects of Sophora flavescens on T helper 1 cell/T helper 2 cell(Th1/Th2)imbalance in atopic dermatitis(AD)and its potential mechanism.Methods Totally 24 female C57BL/6 mice were randomly divided into normal control group,AD group,and AD+Sophora flavescens group by stratified body weight,8 mice in each group.AD mouse model was induced by 2,4-dinitrochlorobenzene/Dermatophagoides farinae extract(DNCB/DFE).HaCaT were divided into three groups:control group,interferon-γ(IFN--γ)/tumor necrosis factor-α(TNF-α)group,and IFN-γ/TNF-α+Sophora flavescens group.Quantitative Real-time polymerase chain reaction(qRT-PCR)was used to detect mRNA expressions of IFN-γ,TNF-α,interleukin-2(IL-2),thymus activation-regulated chemokine(TARC),IL-4,IL-5,and IL-6.The protein expressions of phospho-mitogen activated protein kinase p38(p-p38),p38,phospho-nuclear factor kappa B p65 subunit(p-p65)and p65 in tissues and cells were detected by Western Blot.ELISA was used to detect the release levels of IL-6 and TARC,and the ratio of Th1/Th2 cells in flow cytometry.Results At the cell level:Compared with the control group,IL-6 and TARC mRNA and protein expressions significantly increased(P＜0.01),and expressions of p-p38 and p-p65 proteins were up-regulated(P＜0.01)in IFN-γ/TNF-α group.Compared with the IFN-γ/TNF-α group,IL-6 and TARC mRNA and protein expressions significantly decreased(P＜0.01),and the protein expressions of p-p38 and p-p65 were significantly down-regulated(P＜0.01)in the IFN-γ/TNF-α+Sophora flavescens group.At the animal level,compared with the normal control group,the expression levels of p-p38 and p-p65 protein in the skin tissue significantly increased(P＜0.01),infiltration of inflammatory cells increased in the ear skin tissue,hyperkeratinization and infiltration of mast cells also increased(P＜0.05),and Th2 cells increased in the skin(P＜0.01),expressions of IFN-γ,TNF-α,TARC,IL-6,IL-4,and IL-5 in mouse skin increased(P＜0.01)in the AD model group.Compared with the AD model group,after Sophora flavescens intervention,the expression levels of p-p38 and p-p65 protein decreased in the skin tissue of mice in AD+Sophora flavescens treatment group(P＜0.01).The thickness of epidermis and dermis and the number of mast cells were significantly lowered(P＜0.05).The amount of Th2 cells and the expression levels of TARC,IL-6,IL-4,and IL-5 in mouse tissues significantly decreased in AD+Sophora flavescens treatment group(P＜0.01).Conclusion Sophora flavescens alleviated symptoms of AD possibly by improving the imbalance of Th1/Th2 and inhibiting MAPK/NF-κB signaling pathways.关键词
苦参/特应性皮炎/辅助型T细胞1/辅助型T细胞2失衡/有丝分裂原活化蛋白激酶/核因子κB信号通路/中药Key words
Sophora flavescens/atopic dermatitis/Th1/Th2 imbalance/mitogen-activated protein kinase(MAPK)/NF-κB signaling pathways/Chinese herbal medicine引用本文复制引用
黄盼,潘意,王畅,汪海珍,胡凡..苦参通过抑制MAPK/NF-κB通路改善免疫平衡缓解特应性皮炎实验研究[J].中国中西医结合杂志,2024,44(11):1365-1371,7.基金项目
湖南省自然科学基金青年基金资助项目(No.2023JJ40493) （No.2023JJ40493）
