组织工程与重建外科杂志2024，Vol.20Issue(5)：507-515,9.DOI:10.3969/j.issn.1673-0364.2024.05.002
诱导人脂肪干细胞分化为胰岛素分泌细胞:三种不同诱导分化方案的比较
Generating insulin-producing cells from human adipose-derived stem cells:Comparison of three differentiation protocols
摘要
Abstract
Objective The induced differentiation of adipose stem cells into insulin-secreting cells is a new strategy for the treatment of diabetes mellitus.The efficiency of the induced differentiation schemes reported so far varies greatly.This study aims to compare the differentiation efficiency of three induced differentiation schemes.Methods Fat samples from the same batch were collected to extract fat stem cells for passage culture,and the third-generation fat stem cells were self-aggregated into balls for induction and differentiation in different phases (one-step method,two-step method and three-step method).The expression levels of key genes and proteins in the products of the three schemes at the corresponding stages were detected by PCR and immunofluorescence,and the function of insulin secreting cells was detected by glucose stimulation of insulin secretion experiment.Results Compared with other schemes,the gene expressions of the key transcription factors INS,NKX6.1 and PDX1 in the insulin secreting cells induced by the three-step method were significantly increased,and there were significant differences.Key protein C-peptide and NKX6.1/PDX1 were co-expressed in insulin-secreting cells.Functionally,the insulin secreting cells induced by the three-step method can significantly increase insulin secretion under high glucose stimulation.Conclusion Under the same differentiation conditions,the differentiation efficiency of adipose stem cells induced into insulin secreting cells by the three-step method is superior to that by the two-step method and the one-step method,which provides a basis for establishing an efficient and safe induction differentiation scheme of insulin secreting cells.关键词
胰岛分泌细胞/脂肪干细胞/再生医学/糖尿病Key words
Insulin-producing cells/Adipose-derived stem cells/Regenerative medicine/Diabetes分类
生物科学引用本文复制引用
杨京,刘兴然,尹茜雅,季秋眉,阎羽欣,黄如林,李青峰..诱导人脂肪干细胞分化为胰岛素分泌细胞:三种不同诱导分化方案的比较[J].组织工程与重建外科杂志,2024,20(5):507-515,9.基金项目
国家自然科学基金面上项目(82372535) （82372535）
国家自然科学基金国际(地区)合作与交流项目(82361138568) （地区）
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
