组织工程与重建外科杂志2024，Vol.20Issue(5)：528-537,10.DOI:10.3969/j.issn.1673-0364.2024.05.005
紫外线诱导下的真皮成纤维细胞亚型改变及硅酸锌生物陶瓷对光损伤皮肤的修复作用
Ultraviolet-induced changes in dermal fibroblast subtypes and repair of photodamaged skin by zinc silicate bioceramics
摘要
Abstract
Objective Ultraviolet B (UVB) radiation damages the skin by disrupting the skin barrier function,causing various skin diseases,and facilitating the formation of malignant tumors.Fibroblasts in the dermis are categorized into papillary fibroblasts (Fp) and reticular fibroblasts (Fr),crucial for skin regeneration.The present study elucidates that photodamage leads to alterations in different subtypes of Fb,and addresses this phenomenon to find a therapeutic strategy that can repair photodamage.Methods A mouse model of skin photodamage was established and biological specimens were collected for subsequent analyses,including HE and Masson staining to evaluate tissue structure,DHE staining to detect ROS levels in tissues,γH2AX staining to identify cells with DNA damage,immunofluorescence staining for Lrig1 and Dlk1 to identify distinct Fb subtypes.Furthermore,UVB was used to induce and culture fibroblasts,followed by flow cytometry to detect surface markers,and qPCR to assess mRNA expression related to collagen synthesis and secretion.Lrig1 and DHE,γH2AX fluorescence staining was respectively performed in in vitro assays and in vivo photodamage models by applying ZnCS-configured ionic solutions and microneedle patch interventions loaded with ZnCS.Results In photodamaged skin,the dermis exhibited infiltrative inflammatory cells and disrupted collagen fiber structure with reduced content,accompanied by a significant increase in ROS expression throughout the epidermis and superficial dermis,accompanied by more DNA damage in superficial dermal fibroblasts.Compared to the control group,there was a marked reduction in Lrig1+Fb cells within the photodamaged skin dermis,while no significant difference was detected in Dlk1+Fb cells.Following UVB irradiation of fibroblasts,flow cytometry analysis revealed a substantial decrease in the proportion of Lrig1+Fb cells among the fibroblasts.Additionally,downregulation of COL1A1 expression was noted,alongside upregulation of MMP3 and MMP9 expressions.Finally,silicate bioceramics (ZnCS) promoted Fp proliferation,and ROS generation and damage in photodamaged skin were significantly reduced after intervention with ZnCS.Conclusion Fp reducing is associated with the progression of photodamage to photoaging.Meanwhile,ZnCS,which has a promoting effect on Fp proliferation,exerts a significant repairing effect on photodamaged skin.关键词
硅酸锌生物陶瓷/紫外线B/乳头层成纤维细胞/网状层成纤维细胞/光损伤皮肤/活性氧Key words
Zinc silicate bioceramics/Ultraviolet B/Papillary fibroblasts/Reticular fibroblasts/Photodamaged skin/Reactive oxygen species分类
医药卫生引用本文复制引用
陈方舟,Tan Pohching,周双白,李青峰..紫外线诱导下的真皮成纤维细胞亚型改变及硅酸锌生物陶瓷对光损伤皮肤的修复作用[J].组织工程与重建外科杂志,2024,20(5):528-537,10.基金项目
国家自然科学基金(82272287) （82272287）
上海交通大学医学院附属上海市第九人民医院交叉研究基金(JYJC202215) （JYJC202215）
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
