安徽医科大学学报2024，Vol.59Issue(10)：1713-1719,1728,8.DOI:10.19405/j.cnki.issn1000-1492.2024.10.004
miR-142-5p对口腔鳞状细胞癌及血管生成影响的实验研究
The effect of miR-142-5p on oral squamous carcinoma and in angiogenesis
摘要
Abstract
Objective To investigate the expression of miR-142-5p in oral squamous cell carcinoma(OSCC)tis-sues and cell lines and its effects on oral squamous cell proliferation,migration,invasion and angiogenesis.Meth-ods Sixteen groups of oral tumour tissues and paraneoplastic tissues were collected,and qRT-PCR was applied to detect the expression of miR-142-5p in the tissues.The effects of miR-142-5p on cell proliferation,migration,and invasion were observed by cell counting kit-8(CCK-8),cloning,wound healing,Transwell,invasion assays,and the effect of miR-142-5p on angiogenesis was also detected by lumen formation assay.The expression of angiogene-sisrelated proteins vascular endothelial growth factor(VEGFA),vascular endothelial calreticulin(VE-cadherin),epithelial calreticulin(E-cadherin),matrix metalloproteinase 2(MMP2),and matrix metalloproteinase 9(MMP9)was detected by Western blot after overexpression of miR-142-5p.Results miR-142-5p was lowly ex-pressed in oral tumour tissues and cell lines.CCK-8 and clonogenic assays showed that miR-142-5p was inversely correlated with the proliferation of OSCC cells,wound healing and Transwell assays showed that miR-142-5p was inversely correlated with the migration of OSCC cells,and cell invasion assays showed that miR-142-5p was con-versely correlated with the invasion of OSCC cells.Analysis of lumen formation assay showed that overexpression of miR-142-5p reduced the tube length and nodes of HUVECs.Western blot assay showed that up-regulation of miR-142-5p inhibited the VEGFA,VE-cadherin,MMP2,MMP9 expression and promoted E-cadherin expression.Con-clusion Overexpression of miR-142-5p inhibites the proliferative,migratory and invasive effects of oral squamous carcinoma cells as well as angiogenesis,suggesting that miR-142-5p is a novel target for anti-tumour angiogenesis and against oral squamous carcinoma.关键词
非编码RNA/口腔鳞状细胞癌/miR-142-5p/血管生成/增殖/迁移Key words
non-coding RNA/oral squamous cell carcinoma/miR-142-5p/angiogenesis/proliferation/migration分类
医药卫生引用本文复制引用
刘溢馨,李香玉,邵梦慈,王菁,徐文华,王元银..miR-142-5p对口腔鳞状细胞癌及血管生成影响的实验研究[J].安徽医科大学学报,2024,59(10):1713-1719,1728,8.基金项目
安徽省卫生健康科研项目(编号:AHWJ2022b001) （编号:AHWJ2022b001）
安徽省转化医学研究院科研基金项目(编号:2022zhyx-B03) （编号:2022zhyx-B03）
安徽医科大学基础与临床合作研究提升计划项目(编号:2020xkjT024) （编号:2020xkjT024）
安徽医科大学口腔医学院(附属口腔医院)学科建设"峰原"提升项目(编号:2022xkfyts 04) Health Research Project of Anhui Province(No.AHWJ2022b001) （附属口腔医院）
Research Project of Anhui Provincial Institute of Translational Medicine(No.2022zhyx-B03) （No.2022zhyx-B03）
Basic and Clinical Collaborative Research En-hancement Project of Anhui Medical University(No.2020xkjT024) （No.2020xkjT024）
Discipline Construction"Fengyuan"Collabo-rative Projects of College & Hospital of Stomatology,Anhui Medical University(No.2022xkfyts 04) （No.2022xkfyts 04）
