安徽医科大学学报2024，Vol.59Issue(10)：1759-1768,10.DOI:10.19405/j.cnki.issn1000-1492.2024.10.010
c-Myc调控lncRNA KCTD13-DT对口腔鳞癌的影响
Effects of c-Myc regulation of lncRNA KCTD13-DT on oral squamous carcinoma
摘要
Abstract
Objective To explore the action mechanism of long non-coding RNA(lncRNAs)lncRNA KCTD13-DT in oral squamous cell carcinoma(OSCC)and its potential interaction with transcription factor c-Myc,providing a potential diagnostic and therapeutic target for patients with OSCC.Methods The expression of lncRNA KCTD13-DT in OSCC and paracancerous tissues was detected by qRT-PCR.The effects of c-Myc overexpression and knock-down on human tongue squamous carcinoma cells HN6 and CAL27 were detected by qRT-PCR.Fluorescence in si-tu hybridization(FISH)assessed the localization of lncRNA KCTD13-DT in cells.A dual luciferase reporter gene was used to analyze the role of c-Myc in target binding to the promoter region of lncRNA KCTD13-DT.Stable cell lines with knockdown or overexpression of lncRNA KCTD13-DT were constructed in human OSCC cell lines HN6 and CAL27 by lentiviral infection,and the knockdown and overexpression efficiencies of lncRNA KCTD13-DT were detected by qRT-PCR.Cell proliferation changes were detected by growth curve assay,CCK-8 assay,colony forma-tion assay,and cell migration was detected by scratch assay and Transwell.Results lncRNA KCTD13-DT expres-sion level was reduced in OSCC tissues and OSCC cells(HN6,CAL27),and Western blot verified that after knoc-king down and overexpression of c-Myc in HN6 and CAL27,the qRT-PCR experiments showed that c-Myc nega-tively regulated lncRNA KCTD13-DT,and overexpression of c-Myc significantly down-regulated lncRNA KCTD13-DT;knockdown of c-Myc significantly up-regulated lncRNA KCTD13-DT levels.Dual luciferase reporter gene showed that c-Myc could target lncRNA KCTD13-DT,and c-Myc could be involved in regulating and repressing the transcriptional activity of lncRNA KCTD13-DT.FISH showed that lncRNA KCTD13-DT mainly existed in the nu-cleus.Growth curve assay,CCK-8 assay,cell scratch assay,Transwell,and colony formation assay showed that knockdown of lncRNA KCTD13-DT promoted the growth and proliferation of OSCC cells,and overexpression of ln-cRNA KCTD13-DT significantly inhibited the proliferation and migration of OSCC cells.Conclusion lncRNA KCTD13-DT is negatively regulated by c-Myc.Knockdown of lncRNA KCTD13-DT promotes cell proliferation,while overexpression of it inhibits cell growth.关键词
长链非编码RNA/c-Myc/口腔鳞癌/细胞增殖/细胞迁移Key words
long non-coding RNA/c-Myc/oral squamous cell carcinoma/cell proliferation/cell migration分类
医药卫生引用本文复制引用
李文丽,朱友明,何家才..c-Myc调控lncRNA KCTD13-DT对口腔鳞癌的影响[J].安徽医科大学学报,2024,59(10):1759-1768,10.基金项目
国家自然科学基金项目(编号:81771117) （编号:81771117）
安徽省自然科学基金项目(编号:2308085Y23) National Natural Science Foundation of China(No.81771117) （编号:2308085Y23）
Natural Science Foundation of Anhui Province(No.2308085Y23) （No.2308085Y23）
