安徽医科大学学报2024，Vol.59Issue(10)：1814-1822,9.DOI:10.19405/j.cnki.issn1000-1492.2024.10.017
下颌后缩对大鼠脑血流及脑卒中恢复的影响
The effect of mandibular retrusion on cerebral blood flow and stroke recovery in rats
摘要
Abstract
Objective To explore the changes in cerebral blood flow caused by mandibular retrusion,as well as the impact and potential mechanisms on stroke recovery.Methods 6-week-old SD male rats were selected as experi-mental subjects.The metal cannula was bonded to the rat maxillary incisor for one week,forcing mandibular retru-sion(MR).Cerebral blood flow was detected by laser speckle imaging.Cognitive function was detected by the Morris water.Then,the stroke model was constructed in MR rats by using the middle cerebral artery occlusion(MCAO)method for one week.Meanwhile,metal cannulae were then removed in rats to restore the lower jaw's position(MCAO RO),serving as a positive control group.Consequently,rats were randomly divided into the fol-lowing groups:Sham groups,MCAO groups,MCAO MR groups,and MCAO RO groups.Neurological recovery was assessed through the modified neurological severity score(mNSS).The area of cerebral infarction was evalua-ted by using triphenyltetrazolium(TTC)staining.The changes in nerve cells were observed by using hematoxylin eosin(HE)staining.The protein expression level of vascular endothelial growth factor(VEGF)was detected by immunohistochemistry.The protein expression levels of platelet-endothelial cell adhesion molecule(CD31),sirtuin 6(SIRT6),and thioredoxin interaction protein(TXNIP)were detected by Western blot.The mRNA expression levels of SIRT6,TXNIP,and VEGF were determined by qRT-PCR.Microglia activation marker molecule 1(IBA-1)was detected by immunofluorescence.Resluts Because of mandibular retrusion,laser speckle showed de-creased cerebral blood flow,and the water maze showed decreased cognitive function.Compared to other groups,MCAO MR showed a larger ischemic area in TTC staining,while HE staining and neurological scoring showed poo-rer neurological function recovery.Western blot and qRT-PCR showed that the MCAO MR group inhibited the mR-NA and protein expression levels of SIRT6,upregulated the mRNA and protein expression levels of TXNIP,and in-creased the activation of microglia.Conclusion Mandibular retrusion reduces cerebral blood flow and alters cogni-tive function in rats.Mandibular retrusion inhibits recovery in stroke through the SIRT6/TXNIP axis.关键词
下颌后缩/脑血流/脑卒中/康复/认知功能Key words
mandibular retrusion/cerebral blood flow/stroke/recovery/cognitive分类
医药卫生引用本文复制引用
付春风,刘小郁,韩全成,郑秀云,梅勤,程雨荷,吴婷婷..下颌后缩对大鼠脑血流及脑卒中恢复的影响[J].安徽医科大学学报,2024,59(10):1814-1822,9.基金项目
安徽省高校科研计划项目(编号:2023AH050635) （编号:2023AH050635）
安徽医科大学口腔医学院(附属口腔医院)学科建设"峰原"合作项目(编号:2022xkfyts06、2022xkfyhz03) Scientific Research Project of Universities of Anhui Province(No.2023AH050635) （附属口腔医院）
Discipline Construction"Fengyuan"Collaborative Projects of College & Hospital of Stomatology,Anhui Medical University(Nos.2022xkfyts06,2022xkfyhz03) （Nos.2022xkfyts06,2022xkfyhz03）
