安徽医科大学学报2024，Vol.59Issue(10)：1823-1828,6.DOI:10.19405/j.cnki.issn1000-1492.2024.10.018
来氟米特调控HIF-1α信号通路对类风湿关节炎滑膜细胞自噬的影响
Effect of leflunomide regulating HIF-1α signal pathway on autophagy of synoviocytes in rheumatoid arthritis
摘要
Abstract
Objective To investigate the effect of leflunomide(LEF)on the expression of associated autophagy genes in synoviocytes of rheumatoid arthritis(RA)by regulating HIF-1α signal pathway.Methods Three genera-tions of RA synovial cells were divided into blank control group,LEF group and Tripterygium wilfordii polyglyco-sides group.The blank control group was added with the same volume of DMEM culture medium.The drug group was treated with LEF(concentration 0.2 mg/ml)and Tripterygium wilfordii polyglycosides(concentration 0.03 mg/ml),the proliferation and apoptosis of synovial cells were detected by flow cytometry,the expression of IL-1 β,TNF-α,ANGPTL-4 and VEGF was detected by ELISA,the expression of HIF-1α mRNA was detected by qRT-PCR,and the expression of HIF-1 α,Beclin-1 and BNIP3 protein was detected by Western blot.Results Com-pared with Tripterygium wilfordii polyglycosides group,the expression of IL-1 α,TNF-α,ANGPTL-4 and VEGF in synovial supernatant of LEF group decreased;compared with the blank control group,the expression of HIF-1αmRNA in synovial cells of LEF group and Tripterygium wilfordii polyglycosides group decreased,and the effect of LEF group was the most obvious;compared with the blank control group,the protein expressions of HIF-1α,Bec-lin-1 and BNIP3 in synovial cells of LEF group and Tripterygium wilfordii polyglycosides group decreased,and the effect of LEF group was the most obvious.Conclusion LEF can inhibit the expression of inflammatory factors in RA synovial cells,inhibit HIF-1α signaling pathway,inhibit the expression of autophagy-related genes Beclin-1 and BNIP3,and improve the pathological state of synovitis.关键词
类风湿关节炎/来氟米特/HIF-1α信号通路/自噬/滑膜炎Key words
rheumatoid arthritis/leflunomide/HIF-1α signal pathway/autophagy/synovitis分类
医药卫生引用本文复制引用
兰维娅,马武开,姚血明,蒋总,熊浪,杨淑芬,唐芳..来氟米特调控HIF-1α信号通路对类风湿关节炎滑膜细胞自噬的影响[J].安徽医科大学学报,2024,59(10):1823-1828,6.基金项目
贵州省科技计划项目(编号:黔科合平台人才[2020]2202号、黔科合基础-ZK[2023]一般435) （编号:黔科合平台人才[2020]2202号、黔科合基础-ZK[2023]一般435）
贵州中医药大学国家与省级科技创新人才团队培育项目(编号:贵中医TD合字[2022]004号) （编号:贵中医TD合字[2022]004号）
贵州中医药大学研究生教育创新计划项目(编号:YCXKYB2023006) Scientific and Technological Plan Project of Guizhou Province(Nos.Qiankehe Platform Talent[2020]No.2202,Qiankehe Basic-ZK[2023]General 435) （编号:YCXKYB2023006）
National and Provincial Science and Technology Innovation Talent Team Cultivation Project of Guizhou University of Traditional Chinese Medicine(No.Guizhou Uni-versity of TCM TD Hezi[2022]No.004) （No.Guizhou Uni-versity of TCM TD Hezi[2022]No.004）
Postgraduate Education Innovation Plan Project of Guizhou University of TCM(No.YCXKYB2023006) （No.YCXKYB2023006）
