大豆TIFY基因家族的生物信息学分析及非生物胁迫表达研究OA北大核心CSTPCD

TIFY is a class of plant-specific transcription factors involved in various plant growth and development processes and stress responses.At present,some TIFY genes involved in stress have been reported in Arabidopsis thaliana and rice,but there are still few related studies in soybean.In order to clarify the protein physical and chemical properties,gene structure,promoter cis-acting elements,gene evolution and expression characteristics of soybean TIFY gene family,this study performed bioinformatics analysis,transcriptome data analysis and qRT-PCR verification under drought and salt stress.The result showed:38 soybean TIFY family genes were obtained by bioinformatics method,which were distributed on 16 chromosomes and divided into four subgroups,including 10 pairs of fragment repeat genes and one pair of tandem repeat genes.The results of further analysis showed that all GmTIFY proteins were hydrophilic proteins and the average amino acid sequence length was 280.9 aa.Promoter prediction results showed that there are abundant light signals,hormones and stress in the promoter sequence of GmTIFY genes response-related cis-acting elements.The results of tissue expression pattern analysis showed that there were significant differences in the expression of GmTIFY in many tissues,and the expression level of most genes in all tissues was relatively low or even non-expressed.Through transcriptome data analysis and qRT-PCR experimental verification,it was found that Glyma.01g204400,Glyma.05g235500,Glyma.09g077500,Glyma.13g112000,Glyma.15g179600 and Glyma.15g184900 genes were upregulated under drought and salt stress.These results provide a reference for in-depth research on the functions of soybean TIFY family genes.