大豆科学2024，Vol.43Issue(6)：695-707,13.DOI:10.11861/j.issn.1000-9841.2024.06.0695
大豆磷脂酶D家族基因全基因组鉴定及耐盐性分析
Genome-wide Identification and Salt Tolerance Analysis of Phospholipase D Gene Family in Soybean
摘要
Abstract
Phospholipase D(PLD)and its product phosphatidic acid(PA)play important roles in the regulation of plant growth and development and stress response.Currently,the study of the PLD gene family in soybean is still not comprehensive enough.In order to further search for potential candidate genes related to soybean stress resistance,we used genome-wide analysis to study the gene structure,protein conserved domains,collinearity and phylogenetic relationships of 28 GmPLDs.And the expression levels of genes under salt stress were analyzed by qRT-PCR.The results indicated that GmPLDs were distributed on 16 chromosomes,fragment duplication played an important role in the expansion of the soybean PLD gene family.The gene expression pattern analysis showed that the expression of GmPLDs varied in different tissues,and the promoter cis-acting element analysis showed that GmPLDs have potential functions involved in abiotic stresses and hormonal pathways.The qRT-PCR Analysis showed that 18 GmPLDs expression was significantly up-regulated by salt stress induction,which may be involved in soybean salt tolerance stress.The results provide theoretical basis for the analysis and cloning of soybean salt-tolerant genes,and provide basic information for mining GmPLDs in response to abiotic stress.关键词
大豆/磷脂酶/PLD/基因家族/盐胁迫Key words
soybean/phospholipase/PLD/gene family/salt stress引用本文复制引用
何芳蕾,李兰馨,卢秋连,曹婕,秦君,孔凡江,苏彤,林春..大豆磷脂酶D家族基因全基因组鉴定及耐盐性分析[J].大豆科学,2024,43(6):695-707,13.基金项目
国家自然科学基金(32060421) （32060421）
河北省重点研发计划:现代种业科技创新专项(22326316D). （22326316D）
