大连工业大学学报2024，Vol.43Issue(6)：391-396,6.DOI:10.19670/j.cnki.dlgydxxb.2024.0601
纤维素酶CxGH7A在枯草芽孢杆菌中的分泌表达与酶学性质
Secretory expression and enzymatic properties of cellulase CxGH7A in Bacillus subtilis
摘要
Abstract
CxGH7A gene was obtained from a cellulose degrading bacterium Arthrobotrys sp.CX1,and a recombinant strain pPICZαA-CxGH7A/X-33 of Pichia pastoris has been constructed early before.However,yeast fermentation for expressing exogenous proteins has a longer cycle and higher economic costs.To achieve efficient exogenous expression of CxGH7A,a recombinant plasmid pHT43-CxGH7A was constructed and transferred into Bacillus subtilis WB600 to obtain the recombinant strain pHT43-CxGH7A/WB600.Its enzymatic properties were characterized and fermentation conditions were optimized.The results showed that there was a protein band of 55 ku in the extracellular secretion of the recombinant strain pHT43-CxGH7A/WB600 after expression,and the enzyme activity was 47.25 U/mL.The enzyme activity in the supernatant could reach to 123.73 U/mL in the conditions of TB medium,pH 7.0,induction temperature of 30 ℃,inducer concentration of 0.5 mmol/L and induction time of 36 h after optimization.The efficiency of CxGH7A produced by Bacillus subtilis expression system was 4.6 times higher than that of the Pichia pastoris expression system.关键词
纤维素酶/枯草芽孢杆菌/毕赤酵母/酶学性质Key words
cellulase/Bacillus subtilis/Pichia pastoris/enzymatic properties分类
生物科学引用本文复制引用
李蓉,刘珊君,顾劲博,唐文竹..纤维素酶CxGH7A在枯草芽孢杆菌中的分泌表达与酶学性质[J].大连工业大学学报,2024,43(6):391-396,6.基金项目
国家自然科学基金项目(31600640) （31600640）
辽宁省教育厅基本科研业务费项目(LJKMZ20220886). （LJKMZ20220886）
