高等学校化学学报2024，Vol.45Issue(12)：1-9,9.DOI:10.7503/cjcu20240346
介孔有机硅中空纳米球固定尿酸酶用于尿酸检测
Mesoporous Organosilicon Hollow Nanospheres Immobilized Uricase for the Determination of Uric Acid
摘要
Abstract
Mesoporous organosilicon hollow nanospheres(MONs)were synthesized through a one-step growth-induced etching method,and then uricase was successfully immobilized onto MONs,forming MONs-uircase.Comprehensive characterizations of MONs and MONs-uricase were performed using scanning electron microscopy(SEM),transmission electron microscopy(TEM),infrared spectroscopy,N2 adsorption-desorption measurements,and thermogravimetric analysis(TGA).The results indicated that uricase was successfully immobilized within the mesoporous structure of MONs,with an immobilization efficiency exceeding 90%.MONs possessed excellent adsorp-tion properties due to their mesoporous channels and hollow structures.Compared to free uricase,MONs-uricase exhibited enhanced thermal and pH stability.In the detection of serum uric acid(UA),MONs-uricase demonstrated a good linear relationship within the UA concentration range of 0.01-1.00 mg/mL,along with high specificity.MONs-uricase could be readily recovered through centrifugation,and the recovered MONs-uricase maintained high enzymatic activity after multiple cycles of use,retaining 50%enzymatic activity even after 20 cycles,indicating its excellent reusability.MONs offered promising application prospects in the immobilization and recycling of biological enzymes.关键词
介孔有机硅中空纳米球/尿酸酶固定/尿酸检测Key words
Mesoporous organosilicon hollow nanospheres/Immobilization of uricase/Determination of uric acid分类
化学化工引用本文复制引用
纪赫鸣,张宇航,苗婷婷,王怡倩,于笑蕊,王春艳,王润伟..介孔有机硅中空纳米球固定尿酸酶用于尿酸检测[J].高等学校化学学报,2024,45(12):1-9,9.基金项目
吉林省科技发展计划项目(批准号:20210204008YY,20240101180JC)资助.Supported by the Science and Technology Development Plan Project of Jilin Province,China(Nos.20210204008YY,20240101180JC). （批准号:20210204008YY,20240101180JC）
