高等学校化学学报2024，Vol.45Issue(12)：31-39,9.DOI:10.7503/cjcu20240301
基于石墨烯/金纳米粒子/碳化钛复合材料构建双室酶生物燃料电池自供能葡萄糖生物传感器
A Double-Chamber Enzymatic Biofuel Cells-based Self-powered Glucose Biosensor Based on Graphene/Gold Nanoparticles/Titanium Carbide Nanocomposite
摘要
Abstract
Enzymatic biofuel cells(EBFCs)-based self-powered sensing device has the advantages of simple structure,easy miniaturization,and no need for external power supply.It exhibits potential application prospects in clinical diagnosis,environmental monitoring,biosensing,and other fields.Graphene/gold nanoparticles/titanium carbide(rGO/AuNPs/Ti3C2)nanocomposite modified glassy carbon electrode(GCE)was used as cathode of EBFCs(rGO/AuNPs/Ti3C2/GCE).The bioanode of EBFCs was prepared via immobilization of glucose oxidase(GOx)on the surface of rGO/AuNPs/Ti3C2/GCE.A double-chamber enzymatic biofuel cells-based self-powered glucose biosensor(EBFCs-SPGB)was constructed by combining as-prepared bioanode and cathode in supporting electrolyte separated with a Nafion membrane.In the presence of target glucose,the GOx fixed on the surface of the bioanode promotes an enzymatic reaction.The electrons generated by catalyzing glucose transferred to the cathode through an external circuit,resulting in a reduction reaction on the cathode surface and generating an electrochemical response signal.Due to the excellent conductivity,biocompatibility,and large specific surface area of rGO/AuNPs/Ti3C2 nanocomposite,the synergistic effect of nanocomposite can significantly increase loading amount of GOx and effectively promote electron transfer on the electrode surface.The maximum power output signal of the constructed EBFCs-SPGB shows a good linear relationship with glucose concentration in the range of 0.3-10 mmol/L,with a detection limit of 0.1 mmol/L(S/N=3),which can be applied to analysis of glucose concentration in human serum samples.关键词
葡萄糖/纳米复合材料/酶生物燃料电池/自供能电化学传感器Key words
Glucose/Nanocomposite/Enzymatic biofuel cell/Self-powered electrochemical sensor分类
化学化工引用本文复制引用
李世宣,蒙化,尹学虎,易锦飞,马丽红,张艳丽,王红斌,杨文荣,庞鹏飞..基于石墨烯/金纳米粒子/碳化钛复合材料构建双室酶生物燃料电池自供能葡萄糖生物传感器[J].高等学校化学学报,2024,45(12):31-39,9.基金项目
国家自然科学基金(批准号:21565031,21665027)、云南省科技厅应用基础研究基金(批准号:202001AT070012)和云南民族大学研究生科研创新基金(批准号:2024SKY135)资助.Supported by the National Natural Science Foundation of China(Nos.21665027,21565031),the Applied Basic Research Project of Yunnan Provincial Science and Technology Department,China(No.202001AT070012)and the Graduate Scientific Research Foundation of Yunnan Minzu University,China(No.2024SKY135). （批准号:21565031,21665027）
