高等学校化学学报2024，Vol.45Issue(12)：40-46,7.DOI:10.7503/cjcu20240286
一种热蛋白质组学分析数据的归一化方法
A Data Normalization Method of Thermal Proteome Profiling
摘要
Abstract
We developed a simple,cost-effective method with wide application for the accurate identification of small molecule target proteins,in which indexed retention time(iRT)peptides were adopted to be an internal standard for quantification normalization in label-free quantitative thermal proteome profiling(TPP)analysis.The iRT standard peptides were supplemented into the lysates of 293T cells treated by a well-characterized model drug methotrexate(MTX).These peptides were quite stable during heating under different temperatures and it is reasonable to use them for quantification normalization.The results showed that iRT peptides used as an internal standard provided a good normalization effect on the global proteomes.Meanwhile,the melting curve of the target protein dihydrofolate reductase(DHFR)of MTX has been improved.This method provides an important experimental basis for the implementation and quantitative accuracy of label-free TPP techniques.关键词
非标记定量蛋白质组学/热蛋白质组学分析/索引保留时间/数据归一化Key words
Label-free quantitative proteomics/Thermal proteome profiling/Indexed retention time/Data normalization分类
化学化工引用本文复制引用
甄雪妍,周宝津,刘静雯,任艳..一种热蛋白质组学分析数据的归一化方法[J].高等学校化学学报,2024,45(12):40-46,7.基金项目
国家自然科学基金(批准号:32371500)资助.Supported by the National Natural Science Foundation of China(No.32371500). （批准号:32371500）
