海南医学院学报2024，Vol.30Issue(22)：1690-1696,7.DOI:10.13210/j.cnki.jhmu.20240624.002
木犀草素通过AKT-mTOR通路诱导人卵巢癌A2780细胞凋亡和自噬的研究
A2780 cells luteolin-induced apoptosis and autophagy in human ovarian cancer via AKT-mTOR pathway
摘要
Abstract
Objective:To investigate the effect of luteolin on human ovarian cancer A2780 cells and reveal its possible mecha-nism.Method:The effect of luteolin on the viability of A2780 cells was detected by CCK-8,and the concentration of luteolin was determined;the effects of luteolin on the proliferation and metastasis of A2780 cells were detected by clone spot,scratch,and Transwell assay;flow cytometry was used to detect the changes of apoptosis level under the action of luteolin;the expression of proteins related to apoptosis,autophagy,and AKT-mTOR signaling pathway was detected through Western blot experiments.Re-sults:Luteolin inhibited the viability,proliferation,and metastasis of A2780 cells;the proportion of autophagy related protein LC3-Ⅱ/LC3-Ⅰ increased,the expression of P62 decreased,and the expression levels of P-AKT,P-mTOR,and anti-apoptotic protein Bcl-2 decreased.Conclusion:Luteolin induces apoptosis and autophagy in human ovarian cancer A2780 cells through AKT-mTOR signaling pathway,thereby inhibiting the malignant progression of ovarian cancer cells.关键词
木犀草素/卵巢癌/AKT-mTOR/凋亡/自噬Key words
Luteolin/Ovarian cancer/AKT-mTOR/Apoptosis/Autophagy分类
医药卫生引用本文复制引用
徐如敏,曹雨蝶,连晓芙,蔡烨,石世杰,张帅,张静..木犀草素通过AKT-mTOR通路诱导人卵巢癌A2780细胞凋亡和自噬的研究[J].海南医学院学报,2024,30(22):1690-1696,7.基金项目
This study was supported by the Key Natural Science Research Project of Anhui Provincial Department of Education(KJ2020A0578),and Anhui Province College Student Innovation Training Program(S202210367073,S202310367096) 安徽省教育厅自然科学研究重点项目(KJ2020A0578) （KJ2020A0578）
安徽省大学生创新训练项目(S202210367073,S202310367096) （S202210367073,S202310367096）
