海南医学院学报2024，Vol.30Issue(22)：1697-1707,11.DOI:10.13210/j.cnki.jhmu.20240612.002
尘螨过敏原Der p 7新的IgE抗原表位鉴定及尘螨交叉反应性的研究
Identification of new IgE antigenic epitopes of the dust mite allergen Der p 7,and investigation of dust mite cross-reactivity
摘要
Abstract
Objective:To identify new IgE antigenic epitopes of the dust mite allergen Der p 7,and investigate dust mite cross-reactivity.Methods:ELISA was used to detect the IgE binding of recombinant protein Der p 7 with high clinical relevance for dust mite allergy in dust mite-allergic patients in Hefei City,Anhui Province,East China;new IgE antigenic epitopes of the house dust mite allergen Der p 7 were identified by ELISA and Dot Blot;the structure and thermal stability of Der p 7 and its mu-tants were examined by circular dichroism spectrometry;finally,new cross-allergens of dust mites were identified by ELISA and ELISA inhibition assay to identify new cross-allergens of dust mites.Results:The binding rate of IgE to Der p 7 in dust mite aller-gic patients in Hefei City in East China was found to be 36.2%by ELISA,which was similar to that in Guangzhou City(37.4%)in South China and Spain(34.7%).However,this binding rate was influenced by a variety of factors such as geographic region,natural environment and lifestyle,and was significantly lower compared to Beijing(19.3%)in northern China,Ukraine(22.67%)and Italy(28%),and significantly different compared to Africa(56%)and Japan(more than 60%).Two new IgE binding epitopes of the dust mite major allergen Der p 7 were identified by ELISA and Dot Blot,which were aspartic acid at posi-tion 36 and aspartic acid at position 100,respectively.The structural stability of Der p 7 was demonstrated by circular dichroism spectroscopy experiments with no structural changes after mutation,and its thermal stability characteristics were examined.Finally we newly identified two substances(wheat and peanut)that are cross-sensitised to dust mites using ELISA and ELISA inhibition experiments.Conclusion:Based on the binding rate of the dust mite allergen Der p 7 to the IgE of allergic patients in Hefei City,Anhui Province,East China,the study of new IgE antigenic epitopes provides a theoretical basis for the prevention,diagnosis,and treatment of allergic diseases,and is of reference value for the development of a hypoallergenic vaccine against dust mite aller-gens.The discovery of new dust mite cross-allergens provides a scientific basis for the prevention and treatment of cross-allergic re-actions.关键词
尘螨过敏/Der p 7/IgE结合率/IgE抗原表位/交叉过敏Key words
Dust mite allergy/Der p 7/IgE binding/IgE antigenic epitopes/Cross-allergy分类
医药卫生引用本文复制引用
王璇,程露露,胡婧,马克龙,孙安源,金腾川,陈凤,徐杨书函..尘螨过敏原Der p 7新的IgE抗原表位鉴定及尘螨交叉反应性的研究[J].海南医学院学报,2024,30(22):1697-1707,11.基金项目
This study was supported by Anhui University of Traditional Chinese Medicine Campus Talent Support Project(DT2200000325) （DT2200000325）
Anhui Province Key Research Projects for Universities(RZ2300001713) 安徽中医药大学校级人才支持项目(DT2200000325) （RZ2300001713）
安徽省高校科研重点项目(RZ2300001713) （RZ2300001713）
