高等学校化学学报2024，Vol.45Issue(12)：47-56,10.DOI:10.7503/cjcu20240181
新型稀土近红外荧光探针用于活体多重成像
Novel Rare Earth Near-infrared Fluorescent Probes for in vivo Multiplexed Imaging
摘要
Abstract
Fluorescence encoding and in vivo multiplexed imaging with dual-dimensions of excitation and emission are proposed.A series of second near-infrared(NIR-II,1000-1700 nm)fluorescent probes[α-NaYbF4∶8%Tm@NaYbF4@NaYF4(Yb/Tm),α-NaYbF4∶2%Er@NaYbF4@NaYF4(Yb/Er)and β-NaErF4@NaYF4(Er@Y)]were developed for triple-channel in vivo imaging.Among them,Yb/Tm nanoparticles can emit 1640 nm long-wave second near-infrared fluorescence(NIR-II-L,1500-1900 nm)under 915 nm laser excitation while no fluorescence signal can be detected under 808 nm excitation.Yb/Er can emit NIR-II-L fluorescence at 1532 nm under excitation of 915 nm laser while no fluorescence signal can be detected under 808 nm excitation.In contrast,Er@Y nanoparticles excited by an 808 nm laser can emit NIR-II-L fluorescence at 1525 nm,while a very weak signal can be detected under the excitation of 915 nm.The design of rare earth core-shell nanostructures successfully enhances the fluores-cence intensity by 216 times.Under different power density excitations,the nanoparticles still maintain excellent optical properties of independent excitation/emission.Based on the unique optical properties of these nanoparticles,optical encoding and in vivo high-resolution detection with three channels have been achieved,providing new tools and solutions for optical information storage and in vivo multiplexed analysis.关键词
稀土荧光探针/近红外二区/活体成像/多重成像Key words
Rare earth fluorescent probe/Second near-infrared window/In vivo imaging/Multiplexed imaging分类
化学化工引用本文复制引用
李红雨,张洪新..新型稀土近红外荧光探针用于活体多重成像[J].高等学校化学学报,2024,45(12):47-56,10.基金项目
国家自然科学基金(批准号:22104017)资助.Supported by the National Natural Science Foundation of China(No.22104017). （批准号:22104017）
