河北医学2024，Vol.30Issue(11)：1803-1808,6.DOI:10.3969/j.issn.1006-6233.2024.11.08
曲妥珠单抗对乳腺癌巨噬细胞相关信号因子及预后的影响
Impact of Trastuzumab on Macrophage-Related Signaling Factors and Prognosis in Breast Cancer
摘要
Abstract
Objective:To investigate the impact of trastuzumab on macrophage-related signaling factors and prognosis in human epidermal growth factor receptor 2(HER2)-positive breast cancer.Methods:A total of 155 patients with primary,non-metastatic HER2+breast cancer who underwent surgery between January 2013 and December 2020 were enrolled in this study.Immunohistochemistry was used to analyze the expres-sion levels of CD8+,CD4+,FOXP3+tumor-infiltrating lymphocytes(TILs)and CD68+,CD163+tumor-asso-ciated macrophages(TAMs)in tumor tissues.Spearman correlation analysis was used to assess the correlation between TILs and TAMs.Univariate and multivariate Cox regression analyses were performed to evaluate prog-nostic factors in patients who received adjuvant trastuzumab therapy.Results:Thirty-two patients(20.6%)died during the follow-up period.The median follow-up time for all patients was 53.57 months(range,33.30～72.37).FOXP3+TIL counts were positively correlated with CD4+and CD8+TIL counts(P<0.001).CD4+,CD8+,and FOXP3+TIL counts were positively correlated with CD163+and CD68+TAM counts(P<0.001).Cox regression analysis showed that lymphovascular invasion(OR=2.631,95%CI=1.218-5.685),CD68+TAM(OR=1.002,95%CI=1.001-1.003),CD8+TIL(OR=1.002,95%CI=1.001-1.004),and Ki-67 proliferation index(OR=0.998,95%CI=0.997-1.000)were independent prognostic factors for patients receiving adjuvant trastuzumab therapy(P<0.05).ROC analysis showed that when the cutoff value for CD68+TAM was 235.33,the AUC for predicting patient death was 0.933(95%CI=0.896～0.970),with a sensitivity of 93.8%and a specificity of 83.7%.When the cutoff value for CD8+TIL was 53.67,the AUC for predicting patient death was 0.645(95%CI=0.536-0.754),with a sensitivity of 56.3%and a specificity of 65.0%.Conclusion:High counts of CD68+TAM and CD8+TIL were associated with poor prog-nosis in HER2+breast cancer patients receiving trastuzumab therapy,and CD68+TAM counts had a higher predictive value for adverse prognosis.关键词
曲妥珠单抗/乳腺癌/人类表皮生长因子受体2/肿瘤浸润淋巴细胞/肿瘤相关巨噬细胞Key words
Trastuzumab/Breast cancer/Human epidermal growth factor receptor 2/Tumor-infiltrating lymphocytes/Tumor-associated macrophages引用本文复制引用
刘芳丽,周晓燕..曲妥珠单抗对乳腺癌巨噬细胞相关信号因子及预后的影响[J].河北医学,2024,30(11):1803-1808,6.基金项目
陕西省卫生健康委员会科研项目,(编号:2020131211) （编号:2020131211）
