RNPEP调控巨噬细胞焦亡参与胃癌细胞的生物学行为的分子机制OACSTPCD

Objective:To investigate the effect of RNPEP on the biological behavior of gastric cancer cells and its possible mechanism.Methods:RNPEP mRNA levels in normal gastric tissues and gastric cancer tissues were detected by RT-qPCR.THP-1 cells were cultured and induced into M0 macrophages,which were divided into control group and RNPEP overexpression group.The expression levels of RNPEP,GSDME,NLRP3,IL-1β and IL-18 in macrophages were detected by Western blotting.Human gastric adenocarcinoma cell MGC-803 was co-cultured with M0 macrophages of the control and RNPEP overexpression groups,and divided into control group and RNPEP overexpression group.MGC-803 cells and M0 macrophages in the over-expressing RNPEP group were co-cultured,and IL-1β IgG1 homologous antibody and IL-1β IgG1 neutrali-zing antibody were added to the culture medium of the two groups,respectively,and they were divided into the RNPEP over-expression group and the inhibiting IL-1β group.The proliferation ability of MGC-803 gastric cancer cells was detected by EdU kit.The invasive ability of MGC-803 gastric cancer cells was detected by Transwell assay.The migration ability of MGC-803 gastric cancer cells was detected by scratch test.Results:Compared with normal tissues,mRNA level of RNPEP was increased in gastric cancer tissues(P<0.05).Compared with the control group,the expressions of RNPEP,GSDME,NLRP3,IL-1β,and IL-18 in mac-rophages of the RNPEP over-expression group were increased(P<0.05).Compared with the control group,the proliferation,migration and invasion ability of MGC-803 cells in the RNPEP over-expression group were increased(P<0.05).Compared with the RNPEP over-expression group,the proliferation,migration,and invasion ability of MGC-803 cells in IL-1β inhibition group were decreased(P<0.05).Conclusion:The se-creted protein RNPEP can promote the biological behavior of gastric cancer cells by up-regulating pyroptosis of macrophages.