河北医学2024，Vol.30Issue(11)：1820-1825,6.DOI:10.3969/j.issn.1006-6233.2024.11.011
RNPEP调控巨噬细胞焦亡参与胃癌细胞的生物学行为的分子机制
Molecular Mechanisms of RNPEP-Regulated Macrophage Pyrodeath Involved in the Biological Behaviour of Gastric Cancer Cells
摘要
Abstract
Objective:To investigate the effect of RNPEP on the biological behavior of gastric cancer cells and its possible mechanism.Methods:RNPEP mRNA levels in normal gastric tissues and gastric cancer tissues were detected by RT-qPCR.THP-1 cells were cultured and induced into M0 macrophages,which were divided into control group and RNPEP overexpression group.The expression levels of RNPEP,GSDME,NLRP3,IL-1β and IL-18 in macrophages were detected by Western blotting.Human gastric adenocarcinoma cell MGC-803 was co-cultured with M0 macrophages of the control and RNPEP overexpression groups,and divided into control group and RNPEP overexpression group.MGC-803 cells and M0 macrophages in the over-expressing RNPEP group were co-cultured,and IL-1β IgG1 homologous antibody and IL-1β IgG1 neutrali-zing antibody were added to the culture medium of the two groups,respectively,and they were divided into the RNPEP over-expression group and the inhibiting IL-1β group.The proliferation ability of MGC-803 gastric cancer cells was detected by EdU kit.The invasive ability of MGC-803 gastric cancer cells was detected by Transwell assay.The migration ability of MGC-803 gastric cancer cells was detected by scratch test.Results:Compared with normal tissues,mRNA level of RNPEP was increased in gastric cancer tissues(P<0.05).Compared with the control group,the expressions of RNPEP,GSDME,NLRP3,IL-1β,and IL-18 in mac-rophages of the RNPEP over-expression group were increased(P<0.05).Compared with the control group,the proliferation,migration and invasion ability of MGC-803 cells in the RNPEP over-expression group were increased(P<0.05).Compared with the RNPEP over-expression group,the proliferation,migration,and invasion ability of MGC-803 cells in IL-1β inhibition group were decreased(P<0.05).Conclusion:The se-creted protein RNPEP can promote the biological behavior of gastric cancer cells by up-regulating pyroptosis of macrophages.关键词
胃癌/RNPEP/巨噬细胞焦亡/增殖/侵袭/迁移Key words
Gastric cancer/RNPEP/Macrophage pyroptosis/Proliferation/Invade/Migra-tion引用本文复制引用
霍亚楠,杨晨,郭书丽,路宽,李慎,郭天,薛佳栋..RNPEP调控巨噬细胞焦亡参与胃癌细胞的生物学行为的分子机制[J].河北医学,2024,30(11):1820-1825,6.基金项目
河北省卫生健康委员会科研项目,(编号:20220047) （编号:20220047）
