河北医学2024，Vol.30Issue(11)：1825-1830,6.DOI:10.3969/j.issn.1006-6233.2024.11.012
橄榄苦苷调节JAK2/STAT3信号通路对胆管癌细胞恶性生物学行为的影响
Impacts of Oleuropein on the Malignant Biological Behavior of Cholangiocarcinoma Cells by Regulating the JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective:To investigate the impacts of Oleuropein(Ole)on the malignant biological behav-ior of cholangiocarcinoma cells by regulating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:HuCCT1 cells in logarithmic growth phase were divided into control group,100μg/mL Ole group,200μg/mL Ole group,400μg/mL Ole group,800μg/mL Ole group,and Ole+JAK2/STAT3 signaling pathway activator(Colivelin)group.The control group was not trea-ted,and HuCCT1 cells in 100μg/mL Ole group,200μg/mL Ole group,400μg/mL Ole group,and 800μg/mL Ole groups were treated with 100,200,400,and 800μg/mL Ole,and the Ole+Colivelin group treated HuCCT1 cells with(800μg/mL Ole and 0.5μmoL/L Colivelin respectively).CCK-8 method,plate cloning method,and flow cytometry were applied to determine cell viability,cloning,and apoptosis,respectively.Tr-answell experiment was applied to detect cell migration and invasion.Western blot was applied to determine the expression of JAK2/STAT3 signaling pathway proteins and apoptotic proteins.Results:The HuCCT1 cell viability and number of cloned cells in the 100,200,400,and 800μg/mL Ole groups were lower than those in the control group,and the apoptosis rate was higher than that in the control group,the trend of change was Ole dose-dependent(P<0.05).Compared with the 800μg/mL Ole group,the HuCCT1 cell viability and number of cloned cells in the Ole+Colivelin group increased,while the apoptosis rate decreased(P<0.05).Compared with the control group,the migration and invasion of HuCCT1 cells in the 100,200,400,and 800μg/mL Ole groups showed an Ole dose-dependent decrease(P<0.05).Compared with the 800μg/mL Ole group,the migration and invasion of HuCCT1 cells in the Ole+Colivelin group increased(P<0.05).Compared with the control group,the protein expression of p-JAK2/JAK2,p-STAT3/STAT3,and Bcl-2 in HuCCT1 cells in the 100,200,400,and 800μg/mL Ole groups showed an Ole dose-dependent decrease,the protein expression of Bax showed an Ole dose-dependent increase(P<0.05).Compared with the 800μg/mL Ole group,the expression of p-JAK2/JAK2,p-STAT3/STAT3,and Bcl-2 proteins in HuCCT1 cells in the Ole+Colivelin group increased,the protein expression of Bax decreased(P<0.05).Conclusion:Ole may inhibit the malignant biological behavior of cholangiocarcinoma cells by inhibiting the activation of the JAK2/STAT3 signaling pathway.关键词
橄榄苦苷/Janus激酶2/信号转导和转录激活因子3/胆管癌/恶性生物学行为Key words
Oleuropein/Janus kinase 2/signal transducer and activator of transcription 3/Bile duct cancer/Malignant biological behavior引用本文复制引用
郑伟,韩朝..橄榄苦苷调节JAK2/STAT3信号通路对胆管癌细胞恶性生物学行为的影响[J].河北医学,2024,30(11):1825-1830,6.基金项目
陕西省2023年院级科研课题立项项目,(编号:215KYJJ-202316) （编号:215KYJJ-202316）
