河北医学2024，Vol.30Issue(11)：1761-1766,6.DOI:10.3969/j.issn.1006-6233.2024.11.01
安石榴苷调节PI3K/Akt/mTOR信号通路对急性髓系白血病细胞自噬和凋亡的影响
Effect of Punicalagin on Autophagy and Apoptosis in Acute Myeloid Leukemia Cells by Regulating the PI3K/Akt/mTOR Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of punicalagin(PUN)on autophagy and apoptosis in a-cute myeloid leukemia(AML)cells by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway.Methods:Human AML cells(HL-60)were cultured in vitro and randomly separated into control group(normal culture),L-PUN,M-PUN,H-PUN groups(20,40,60 μg/mL PUN),and 740 Y-P group(60 μg/mL PUN+30 μmol/L PI3K/Akt/mTOR signaling pathway activator 740 Y-P).Methylthiazolyltetrazolium(MTT)method and plate cloning method were applied to detect cell proliferation in each group.MDC staining was applied to detect the occur-rence of autophagy of cells in various groups.Flow cytometry was applied to detect cell apoptosis in each group.Protein immunoblotting(WB)was applied to detect the expression of apoptosis related proteins(Bcl-2,Bax),autophagy related proteins(P62,LC3-II/I ratio,BECN1),and PI3K/Akt/mTOR signaling path-way related proteins(p-PI3K,p-Akt,p-mTOR)of cells in each group.Results:Compared with the control group,the survival rate,clone count,p-PI3K,p-Akt,p-mTOR,P62,and Bcl-2 protein expression of HL-60 cells in the L-PUN group,M-PUN group,and H-PUN group decreased,while the apoptosis rate,auto-phagosome positive rate,LC3-II/I ratio,BECN1,and Bax protein expression increased(P<0.05).Com-pared with the H-PUN group,the survival rate,clone number,p-PI3K,p-Akt,p-mTOR,P62,and Bcl-2 protein expression of the 740 Y-P group cells increased,while the apoptosis rate,autophagosome positive rate,LC3-II/I ratio,BECN1,and Bax protein expression levels decreased(P<0.05).Conclusion:PUN inhibits the PI3K/Akt/mTOR signaling pathway,inhibits HL-60 cell proliferation,promotes apoptosis and autophagy,and slows down the progression of AML.关键词
安石榴苷/磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路/急性髓系白血病/细胞凋亡/自噬Key words
Punicalagin/Phosphatidylinositol 3-kinase/protein kinase B/mammalian target of ra-pamycin signaling pathway/Acute myeloid leukemia/Cell apoptosis/Autophagy引用本文复制引用
常凤,张琳琳..安石榴苷调节PI3K/Akt/mTOR信号通路对急性髓系白血病细胞自噬和凋亡的影响[J].河北医学,2024,30(11):1761-1766,6.基金项目
河北省中医药管理局科研计划项目,(编号:2019538) （编号:2019538）
