河北医学2024，Vol.30Issue(11)：1766-1772,7.DOI:10.3969/j.issn.1006-6233.2024.11.02
金圣草黄素抑制IL-6/JAK2/STAT3通路抑制人胃癌裸鼠原位移植瘤生长和转移
Chrysoeriol Inhibits the Growth and Metastasis of Orthotopic Grafted Human Gastric Cancer in Nude Mice by Inhibiting IL-6/JAK2/STAT3 Pathway
摘要
Abstract
Objective:To investigate the effects of Chrysoeriol(Chry)on the growth and metastasis of human gastric cancer in nude mice and the IL-6/JAK2/STAT3 signaling pathway.Methods:Orthotopic transplantation model of human gastric cancer nude mice was established,and successfully modeled nude mice were randomly separated into a model group,low,medium,and high dose Chry groups(Chry-L,Chry-M,Chry-H groups),and high-dose Chry+JAK2 activator Coumermycin A1 group(Chry-H+CA1 group),12 animals per group.After subcutaneous injection,the nude mice were killed to take transplanted tumors and weigh them,and the tumor inhibition rate was calculated.HE staining was applied to observe the pathological changes of transplanted tumor tissue.TUNEL staining was applied to detect apoptosis in transplanted tumor tis-sues.Immunohistochemistry was applied to detect the expression of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)proteins related to migration and invasion of transplanted tumors.Immunoblotting was applied to detect apoptosis of transplanted tumor cells and the expression of IL-6/JAK2/STAT3 signaling pathway related proteins.Results:Compared with the model group,the tumor cell density in the Chry-L,Chry-M,and Chry-H groups decreased,with loose and disordered arrangement,a large number of tumor cells ruptured and necrotic,obvious proliferation of connective tissue,with an increase in nuclear py-knosis and deep staining,a decrease in nuclear division,an increase in apoptotic and necrotic cells,and a re-duction in the quality of transplanted tumors,the tumor inhibition rate,cell apoptosis index,and Bax expres-sion increased,the tumor metastasis rate and the expression of Bcl-2,MMP-2,MMP-9,IL-6,p-JAK2/JAK2,p-STAT3/STAT3 reduced,the Chry-H group showed the most obvious changes(P<0.05).Com-pared with the Chry-H group,the Chry-H+CA1 group showed an increase in tumor cells,relatively neat ar-rangement,reduced nuclear pyknosis and deep staining,increased nuclear division,obvious tumor necrosis,and increased weight of transplanted tumors,the tumor inhibition rate,cell apoptosis index,and Bax expres-sion decreased,the tumor metastasis rate and the expression of Bcl-2,MMP-2,MMP-9,IL-6,p-JAK2/JAK2,and p-STAT3/STAT3 increased(P<0.05).Conclusion:Chrysoeriol can inhibit the growth and me-tastasis of human gastric cancer transplanted in nude mice,and its mechanism of action is related to the inhi-bition of the IL-6/JAK2/STAT3 signaling pathway.关键词
金圣草黄素/IL-6/JAK2/STAT3信号通路/胃癌/移植瘤/转移Key words
Chrysoeriol/IL-6/JAK2/STAT3 signaling pathway/Gastric cancer/Transplanted tumor/Metastasis引用本文复制引用
刘霞,杨东,张朵朵,刘晓蕾,马立东..金圣草黄素抑制IL-6/JAK2/STAT3通路抑制人胃癌裸鼠原位移植瘤生长和转移[J].河北医学,2024,30(11):1766-1772,7.基金项目
2022年度河北省医学科学研究课题计划,(编号:20220491) （编号:20220491）
