河北医学2024，Vol.30Issue(11)：1772-1778,7.DOI:10.3969/j.issn.1006-6233.2024.11.03
金合欢素调节NF-κB信号通路对幽门螺旋菌感染小鼠胃黏膜损伤的研究
The Effect of Acacetin on Gastric Mucosal Injury in Helicobacter Pylori-Infected Mice by Regulating NF-κB Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of Acacetin on gastric mucosal injury in Helicobacter py-lori(Hp)-infected mice by regulating NF-κB signaling pathway.Methods:C57BL/6 mice were given 109 CFU/mL Hp(0.3 mL)gavage,and the successfully modeled mice were randomly divided into Hp group,low Acacetin(Acacetin-L,30 mg/kg),high dose(Acacetin-H,75 mg/kg)group,positive control[ome-prazole(400 μg/kg),clarithromycin(7.15 mg/kg)and amoxicillin(14.25 mg/kg)]group,and Acacetin-H+Prostratin(75 mg/kg Acacetin+0.5 mg/kg Prostratin)group,and mice given 0.3 mL of normal saline by gavage were made the control group.Gastrointestinal hormones[motilin(MTL),Ghrelin,5-tryptamine(5-HT)]in serum were measured.Gastric tissue was isolated and the pathological changes and scores were detected.The levels of interleukin(IL)-1β,IL-10,tumor necrosis factor(TNF)-α,mRNA expression of occludin(Occludin),atresia band protein(ZO-1),apoptosis and the expression of apoptotic proteins Bax,Bcl-2,p-NF-kB p65 and NF-kB p65 were detected.Results:Compared with the control group,the Ghre-lin level,IL-10 level,Occludin,ZO-1 mRNA expression and Bcl-2 expression in the Hp group were greatly reduced,the MTL,5-HT levels,damage score,IL-1β,TNF-α levels,apoptosis rate,Bax,p-NF-kB p65/NF-kB p65 expression were greatly increased(P<0.05).Compared with the Hp group,the Ghrelin lev-el,IL-10 level,Occludin,ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-L,Acacetin-H group,positive control group were greatly increased,the MTL,5-HT levels,damage score,IL-1β,TNF-α levels,apoptosis rate,Bax,p-NF-kB p65/NF-kB p65 expression were greatly reduced,there were differ-ences among different Acacetin groups(P<0.05).Compared with the Acacetin-H group,the Ghrelin level,IL-10 level,Occludin,ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-H+Prostratin group were greatly decreased,the MTL,5-HT levels,damage score,IL-1β,TNF-α levels,apoptosis rate,Bax,p-NF-kB p65/NF-kB p65 expression were greatly increased(P<0.05).Conclusion:Acacetin can improve gastric mucosal injury in Hp infected mice through NF-κB signaling pathway.关键词
金合欢素/NF-κB信号通路/幽门螺旋菌/胃黏膜Key words
Acacetin/NF-κB signaling pathway/Helicobacter pylori/Gastric mucosa引用本文复制引用
梁磊,贾纯亮,李青科,马国明,王剑,李翰嵩,张磊..金合欢素调节NF-κB信号通路对幽门螺旋菌感染小鼠胃黏膜损伤的研究[J].河北医学,2024,30(11):1772-1778,7.基金项目
河北省医学科学研究课题计划项目,(编号:20201528) （编号:20201528）
