河北医学2024，Vol.30Issue(11)：1797-1802,6.DOI:10.3969/j.issn.1006-6233.2024.11.07
七氟醚调节Nrf2/HO-1信号通路对肺结核大鼠肺组织损伤的影响
Effect of Sevoflurane on Lung Tissue Damage in Rats with Pulmonary Tuberculosis by Regulating the Nrf2/HO-1 Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of sevoflurane(Sevo)on lung tissue damage in pulmona-ry tuberculosis(PTB)rats by regulating the nuclear factor E2 related factor(Nrf2)/heme oxygenase(HO-1)signaling pathway.Methods:A PTB rat model was established.The rats were separated into control group,model group,high-dose,medium-dose,and low-dose sevoflurane groups(S-H group,S-M group,S-L group),and high-dose sevoflurane+Nrf2 inhibitor group(S-H+ML385 group).The levels of inflamma-tory factors and oxidative stress in serum were detected,the pathological changes in rat lung tissue were ob-served and the number of tuberculosis bacteria was calculated,and protein expression was detected.Results:Compared with the control group,the model group showed more severe lung tissue damage,with a large num-ber of tuberculosis nodules and inflammatory cell infiltration,and disordered alveolar structure,the number of tuberculosis bacterial colonies increased,and the levels of IL-6,TNF-α,IL-1β,MDA and ROS increased,the level of SOD decreased,and the expression of Nrf2 and HO-1 was down regulated(P<0.05).Compared with the model group,the lung tissue damage was improved in the S-L,S-M,and S-H groups,the number of tuberculosis bacterial colonies decreased,the levels of IL-6,TNF-α,IL-1β,MDA,and ROS decreased,the level of SOD increased,and the expression of Nrf2 and HO-1 was upregulated(P<0.05).Compared with the S-H group,the lung tissue damage in the S-H+ML385 group worsened,the number of tuberculosis bacterial colonies increased,and the levels of IL-6,TNF-α,IL-1β,MDA,and ROS increased,the level of SOD decreased,and the expression of Nrf2 and HO-1 was down regulated(P<0.05).Conclusion:Sevoflu-rane can improve lung tissue damage in rats with pulmonary tuberculosis,possibly by activating the Nrf2/HO-1 signaling pathway.关键词
肺结核/七氟醚/核因子E2相关因子/血红素氧化酶/肺组织损伤Key words
Pulmonary tuberculosis/Sevofluoroether/Nuclear factor E2 related factor/heme oxy-genase/Lung tissue damage引用本文复制引用
张湲钫,张芳龄,张立..七氟醚调节Nrf2/HO-1信号通路对肺结核大鼠肺组织损伤的影响[J].河北医学,2024,30(11):1797-1802,6.基金项目
陕西省卫生健康科研基金项目,(编号:2021D027) （编号:2021D027）
