解剖学杂志2024，Vol.47Issue(5)：410-414,429,6.DOI:10.3969/j.issn.1001-1633.2024.05.008
槐耳多糖调节Rho/ROCK信号通路对结肠癌SW620细胞恶性生物学行为的影响
Impacts of Huaier polysaccharide on the malignant biological behavior of SW620 cells of colon cancer by regulating the Rho/ROCK signaling pathway
摘要
Abstract
Objective:To investigate the impacts of Huaier(trametes robiniophila murr)polysaccharide on the malignant biological behavior of SW620 cells of colon cancer by regulating Rho/Rho-associated coiled-coil kinase(ROCK)signaling pathway.Methods:Human colon cancer cell line SW620 was divided into control group,Huaier polysaccharide group,narciclasine(Rho/ROCK pathway activator)group,and Huaier polysaccharide+narciclasine group.Cell proliferation,migration,invasion,apoptosis,and colony-forming ability of the cells in each group were analyzed.The expression levels of RhoA,ROCK1,nuclear associated antigen(Ki67),matrix metalloproteinase 2(MMP2),and caspase-3 proteins were detected by Western blotting.The model of colon cancer was established in nude mice to verify the effect of Huaier polysaccharide on the growth of transplanted colon cancer in nude mice.Results:Huaier polysaccharide inhibited SW620 cell proliferation,migration,invasion,and colony formation and increased apoptosis as well as RhoA,ROCK1,Ki67,and MMP2 protein expression levels.Narcocycline reversed the inhibitory effect of Huaier polysaccharide on the malignant biological behavior of SW620 cells.Conclusion:Huaier polysaccharide inhibits the malignant biological behavior of SW620 cells by inhibiting the Rho/ROCK signaling pathway.关键词
槐耳多糖/Rho/ROCK信号通路/结肠癌细胞Key words
Huaier polysaccharide/Rho/ROCK signaling pathway/colon cancer cell分类
医药卫生引用本文复制引用
温秀英,陈战,黄军利,陈洪标,林茜,温志坚..槐耳多糖调节Rho/ROCK信号通路对结肠癌SW620细胞恶性生物学行为的影响[J].解剖学杂志,2024,47(5):410-414,429,6.基金项目
厦门市医疗卫生指导性项目(3502Z20214ZD1187) （3502Z20214ZD1187）
