解剖学杂志2024，Vol.47Issue(5)：420-424,5.DOI:10.3969/j.issn.1001-1633.2024.05.010
γ-亚麻酸对血管性认知障碍的作用及其机制
Effect of γ-linolenic acid on vascular cognitive impairment and its mechanism
摘要
Abstract
Objective:To explore the effect and mechanism of gamma-linolenic acid(GLA)on vascular cognitive impairment(VCI).Methods:Thirty male C57BL6J mice were evenly divided into control group,VCI group,and GLA group.The VCI mouse model was established by bilateral carotid artery ligation in the VCI group and GLA group,and the sham operation was performed in the control group.The VCI group and sham group were given 1 mg/kg normal saline daily,while the GLA group was given 1 mg/kg GLA daily.14 days later,the Morris water maze was used to detect the level of cognitive impairment of mice.The mice were sacrificed and the brain tissue of mice was observed by H-E staining.The neurons in the hippocampus of mice were observed by transmission electron microscopy.Western blotting was used to detect the expression levels of PI3K/AKT/mTOR pathway-related proteins,autophagy-related proteins,and neuronal apoptosis-related proteins in mice.Results:The escape latency of mice in the VCI group was longer than that in the GLA group and control group,and the number of platform crossings and the residence time in the target quadrant were shorter than those in the other two groups.The morphology of neurons in the GLA group was better than that in the VCI group,but worse than that in the control group.The expression levels of PI3K/AKT/mTOR pathway-related proteins,autophagy-related proteins,and neuronal apoptosis-related proteins in the VCI group were higher than those in the control group and GLA group,with the GLA group showing higher levels than the control group.Conclusion:GLA mitigates neuronal apoptosis in treating VCI by suppressing autophagy via the PI3K/AKT/mTOR pathway.关键词
γ-亚麻酸/认知障碍/血管性痴呆/氧化应激Key words
γ-linolenic acid/cognitive impairment/vascular dementia/oxidative stress分类
医药卫生引用本文复制引用
石剑,贡丽雅,王燕..γ-亚麻酸对血管性认知障碍的作用及其机制[J].解剖学杂志,2024,47(5):420-424,5.基金项目
河北省医学科学研究课题计划(20200953) （20200953）
