南京林业大学学报（自然科学版）2024，Vol.48Issue(6)：51-61,11.DOI:10.12302/j.issn.1000－2006.202404005
鹅掌楸LcPIN 1a基因的克隆及其对植株生长发育的影响
Cloning of the Liriodendron chinense LcPIN 1a genes and its effect on plant growth and development
摘要
Abstract
[Objective]This study aimed to explore the role of the auxin transporter PIN1 in plant growth and development in Liriodendron chinense.[Method]Three PIN1 homologous proteins were identified in the Liriodendron genome using bioinformatic methods,and expression pattern analyses of the three LcPIN 1s genes were performed in different tissues and response to various abiotic stresses.An overexpression vector driven by the CaMV35S promoter was then constructed and transformed into Arabidopsis and Liriodendron×sinoamericanum,followed by phenotypic determination of growth and developmental traits in the transgenic positive lines.[Result]Three PIN1 homologous proteins were identified in the Liriodendron genome,named LcPIN1a,LcPIN1b and LcPIN1c.Expression pattern analyses showed that LcPIN 1a was mainly expressed in leaves,while LcPIN 1b and LcPIN 1c were primarily expressed in roots and stems and stigmas when the plantlets transitioned into reproductive growth.In addition,all three LcPIN 1genes transcriptionally responded to drought stress,with LcPIN 1b and LcPIN 1c showing dependence on the biosynthesis of endogenous ABA,while LcPIN 1a does not.Root length and plant height were significantly reduced in LcPIN 1a-heterologous overexpression(LcPIN 1a-HO)lines compared to wild-type Arabidopsis.The number of stamens was predominantly five in LcPIN 1a-HO lines,whereas wild-type Arabidopsis typically contained six stamens.The regeneration of plantlets in LcPIN 1a-overexpressing(LcPIN 1a-OE)Liriodendron×sinoamericanum was significantly reduced compared to wild-type plants.In addition,the root length and plant height of LcPIN 1a-OE regenerated seedlings were significantly lower than those of the wild type.The root structure of LcPIN 1a-OE plants was significantly changed,with the taproot being less distinct.[Conclusion]The PIN1 proteins of L.chinense play a crucial role in vegetative and reproductive growth.Overexpression of LcPIN 1genes can be detrimental to normal plant growth and development.关键词
鹅掌楸/生长素转运蛋白/PIN1基因/生长发育Key words
Liriodendron chinense/auxin transporter/PIN 1gene/growth and development分类
农业科技引用本文复制引用
郝兆东,马筱筱,王丹丹,陆叶,施季森,陈金慧..鹅掌楸LcPIN 1a基因的克隆及其对植株生长发育的影响[J].南京林业大学学报（自然科学版）,2024,48(6):51-61,11.基金项目
国家自然科学基金面上项目(32071784). （32071784）
