水生生物学报2024，Vol.48Issue(12)：1965-1979,15.DOI:10.7541/2024.2024.0199
鳡幼鱼肌纤维发育特征及驯食策略对肌肉发育的影响
DEVELOPMENT CHARACTERISTICS OF MUSCLE FIBER IN JUVENILE ELOPICHTHYS BAMBUSA AND THE EFFECTS OF WEANING STRATEGIES ON MUSCLE DEVELOPMENT
摘要
Abstract
Skeletal muscle comprises 30%—80%of fish body weight,which is very important for the health and economic value of fish.Elopichthys bambusa is a high-value economic fish,but the molecular mechanisms underlying muscle development in its juveniles remain unclear.In this study,the muscle development characteristics of juvenile Elopichthys bambusa at different developmental stages and under different domestication strategies were discussed by using histological and transcriptome analysis,complemented by fluorescence quantitative PCR verification.The results showed that the mosaic proliferation of muscle fibers of Elopichthys bambusa larvae began on the 22nd day post-hatch-ing(dph).During the early stage of muscle mosaic proliferation,genes related to MyoGenic regulators(myog and myod),the myostatin gene(mstn),muscle satellite cell marker gene(pax7),and muscle differentiation-related genes(myl3,tnni2,myoz2,tpm1)were actively involved.In addition,the use of artificial compound feed can improve muscle metabolism and promote growth in juvenile Elopichthys bambusa,with the 22 dph group showing higher weaning effi-ciency and better weaning effect.This study provides a foundation for understanding the characteristics and molecular mechanism of muscle development in Elopichthys bambusa and provide a theoretical reference for large-scale farming practices.关键词
骨骼肌/马赛克增生/肌肉转录组/驯食/鳡Key words
Skeletal muscle/Mosaic proliferation/Muscle transcriptome/Weaning/Elopichthys bambusa分类
农业科技引用本文复制引用
龙小波,高泽霞,何焱,管赫赫,曹靖花,丁洋,胡晨昊,别汉晴,梁浩文,任旌雷,沈志刚..鳡幼鱼肌纤维发育特征及驯食策略对肌肉发育的影响[J].水生生物学报,2024,48(12):1965-1979,15.基金项目
国家自然科学基金联合基金(U21A20263) （U21A20263）
湖北省重点研发计划(2021BBA233)资助[Supported by the Joint Fund Project of National Natural Science Foundation of China(U21A20263) （2021BBA233）
Key R&D Program of Hubei Province(2021BBA233)] （2021BBA233）
