水生生物学报2024，Vol.48Issue(12)：1980-1992,13.DOI:10.7541/2024.2024.0102
驯食策略对鳡幼鱼存活、生长、肠道组织学与消化酶及肝脏转录组的影响
WEANING STRATEGY ON SURVIVAL,GROWTH,INTESTINAL HISTOLOGY,ENZYME ACTIVITY,AND LIVER TRANSCRIPTOME OF JUVENILE YELLOWCHEEK CARP ELOPICHTHYS BAMBUSA
摘要
Abstract
The yellowcheek carp(Elopichthys bambusa)is a large and aggressive carnivorous fish.Weaning represents a significant bottleneck in the large-scale aquaculture of yellowcheek carp.To investigate the appropriate timing for weaning and its effects on growth and intestinal health,15 days post-hatching(dph)weaning group(W15),a 22 dph weaning group(W22),and a 29 dph weaning group(W29)were set up to wean yellowcheek carp at 15 dph,22 dph,and 29 dph,respectively.We investigated the effects of weaning starting time on growth,survival,and intestinal deve-lopment.The results showed that the total length,body weight,and survival rate of juvenile yellowcheek carp in the W22 and W29 groups were significantly higher than that in the W15 group at different periods.Additionally,intestinal villus height and muscle thickness were significantly higher in the W22 and W29 groups compared to the W15 group.At 36 dph,the W22 group exhibited the highest specific growth rate,while the W29 group had the highest survival rate.During the weaning period,when the prey's body depth equaled the predator's mouth width,the predator's total length was approximately 1.5 times the prey's total length,described by the relationship TLprey=0.53×TLpredator+3.63.More-over,the trypsin activity in the fast-growing(FB)group of juvenile yellowcheek carp was significantly higher than that in the slow-growing(FS)group,whereas the activity of amylase,lipase,and alkaline phosphatase were significantly lower.Deferentially expressed genes in the FB and FS groups were significantly enriched in lipid metabolic pathways,including the PPAR signaling pathway,fatty acid metabolism,and fatty acid biosynthesis.qPCR confirmed significant differences in the expression of four lipid metabolism-related genes:FABP7,FAS,CPT1,and GYS2.The results indi-cated that initiating weaning at 22-29 dph can obtain the best growth and survival outcomes.Additionally,selecting larger individuals,approximately 1.5 times the average length of the population,can prevent cannibalism and improve survival rate during weaning.The growth performance of juvenile yellowcheek carp post-weaning may be linked to differences in intestinal digestive enzyme activities and lipid metabolism ability.This study provides important gui dance for the weaning and post-weaning cultivation of juvenile yellowcheek carp and may inform weaning research for other carnivorous fish species.关键词
苗种培育/驯食/肠道健康/消化酶/残食/鳡Key words
Seedling rearing/Weaning/Intestinal health/Digestive enzyme/Cannibalism/Elopichthys bambusa分类
农业科技引用本文复制引用
管赫赫,高泽霞,樊启学,沈志刚,龙小波,丁洋,胡晨昊,别汉晴,梁浩文,任旌雷,曹靖花,何焱..驯食策略对鳡幼鱼存活、生长、肠道组织学与消化酶及肝脏转录组的影响[J].水生生物学报,2024,48(12):1980-1992,13.基金项目
湖北省重点研发计划(2021BBA233)资助[Supported by the Key R&D Program of Hubei Province(2021BBA233)] （2021BBA233）
