水生生物学报2024，Vol.48Issue(12)：1993-2005,13.DOI:10.7541/2024.2024.0232
胰酶对大口黑鲈幼鱼生长、肝脏与肠道健康的影响
PANCREATIN ON GROWTH PERFORMANCE,LIVER,AND INTESTINAL HEALTH OF JUVENILE LARGEMOUTH BASS(MICROPTERUS SALMOIDES)
摘要
Abstract
In order to evaluate the effects of different levels of pancreatin(from porcine pancreas)on the growth,diges-tion,liver and intestinal health of juvenile largemouth bass(Micropterus salmoides),a study was conducted where 40%of fish meal protein was replaced with cottonseed meal.Pancreatin was added at levels of 250,500,750,1000,and 1250 mg/kg levels(TG-250,TG-500,TG-750,TG-1000 and TG-1250,respectively),ensuring isonitrogenous and isolipidous conditions.A normal commercial diet served as the negative control group(NCG),and the cottonseed meal replacement(without pancreatin)group served as the positive control group(PCG).The juvenile largemouth bass[initial body weight(8.00±0.50)g]was fed for 70d.The results showed that replacing fish meal with cottonseed meal decreased feed utilization rate,growth,and digestive performance(P<0.05),and adversely affected liver and intestinal health(P<0.05).However,adding pancreatin significantly increased final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and survival rate(SR),and decreased the feed coefficient(P<0.05)in the range of 250-750 mg/kg.At 750 mg/kg,the condition factor(CF)increased(P<0.05),while the visceral index(VSI)and hepatosomatic index(HSI)decreased(P<0.05),and crude protein content increased(P<0.05).The activities of protease,amylase,and lipase in the stomach,foregut,and liver increased in the range of 250-1000 mg/kg(P<0.05).At 750 mg/kg pancreatin,the apparent digestibility of dry matter,crude protein,and crude fiber was the highest,and the nitrogen and phosphorus content in feces was the lowest.In the range of 250-750 mg/kg,malondialdehyde(MDA)levels decreased(P<0.05),while catalase(CAT),total superoxide dismutase(T-SOD),total antioxidant capacity(T-AOC),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)activities increased(P<0.05).At 750 mg/kg,the relative expression of sod and cat gene in liver was up-regulated(P<0.05),the relative expression of anti-inflammatory factors IL-10 and TGF-β was the highest,while the relative expression of pro-inflammatory factors IL-1β,IL-8,and TNF-a was the lowest.Additionally,the expressions of occludin,zo-1,and claudin-1 in the intestinal tract was up-regulated(P<0.05),and the activities of diamine oxidase(DAO),D-lactic acid(D-Lac),and endotoxin(ET)in serum were decreased(P<0.05).In conclusion,the replacement of 40%fish meal protein by cottonseed meal had a significant effect on the growth and health of juvenile largemouth bass.The growth performance,digestive enzyme activity,and apparent digestibility could be significantly improved by adding appropriate amount of pancreatin(750 mg/kg),as well as the liver and intestinal health.Therefore,pancreatin can be used as a high-quality additive for juvenile largemouth bass feed.关键词
胰酶/生长/消化/肝功能/肠道健康/大口黑鲈Key words
Pancreatin/Growth performance/Digestion performance/Liver function/Intestinal health/Micropterus salmoides分类
农业科技引用本文复制引用
刘崇万,朱晓华,王明华,张娇,蔡青和,徐志华,任娣,李昊泽..胰酶对大口黑鲈幼鱼生长、肝脏与肠道健康的影响[J].水生生物学报,2024,48(12):1993-2005,13.基金项目
江苏现代农业(大宗鱼类)产业技术体系建设项目(JATS[2022]371和JATS[2023]334) （大宗鱼类）
国家特色淡水鱼产业技术体系(CARS-46)南京综合试验站资助[Supported by the Jiangsu Agricultural Industry Technology System(JATS[2022]371 and JATS[2023]334) （CARS-46）
Agriculture Research System of China(CARS-46)Nanjing Comprehensive Experimental Station] （CARS-46）
