水生生物学报2024，Vol.48Issue(12)：2006-2017,12.DOI:10.7541/2024.2024.0130
似鮈肠道菌群多样性及其潜在益生菌的筛选鉴定
INTESTINAL MICROBIOTA DIVERSITY AND THE SCREENING AND IDENTI-FICATION OF POTENTIAL PROBIOTICS IN PSEUDOGOBIO VAILLANTI
摘要
Abstract
In order to understand the diversity of intestinal microbiota in Pseudogobio vaillanti and their potential as probiotics,high throughput sequencing technology was used to analyze the 16S rRNA gene of intestinal microbiota.Furthermore,the bacteriostatic effect,enzyme production capability,tolerance,and safety of the isolated strains were determined to identify potential probiotics,followed by strain identification.The results showed that Firmicutes,Bacteroidota,and Proteobacteria were the dominant phylum in the intestinal tract of Pseudogobio vaillanti,accounting 79.98%of the total microbiota.The dominant classes included Bacteroidia,Clostridia,Bacilli,and Gammaproteobacte-ria,accounting for 74.69%.The top 10 genera with the highest relative abundance were Lactobacillus,Akkermansia,Clostridium_sensu_stricto,Acinetobacter,Bacteroides,Dubosiella,UCG-005,Lachnospiraceae_NK4A136_group,Rikenellaceae_RC9_gut_group,and Mycoplasma,collectively accounting for 28.77%.Two strains,S4 and S5,exhibi-ted notable antibacterial and enzyme-producing effects.These strains exhibited survival rates greater than 57%in artifi-cial gastric fluid with pH levels ranging from 2.0 to 6.0,artificial intestinal fluid with pH 6.8,and 0.3%to 0.5%bile salts.They showed no drug resistance or hemolytic activity and were identified as Bacillus velezensis.Therefore,the intestinal microbiota of Pseudogobio vaillanti is diverse,and Bacillus velezensis strains S4 and S5 are promising probio-tics due to their antibacterial properties,robust enzyme production,tolerance,and high safety performance.This study provides a solid foundation for further research into fish intestinal microbiota diversity and offers a theoretical basis for the development of probiotics and healthy aquaculture practices.关键词
芽孢杆菌/肠道菌群/益生菌/似鮈Key words
Bacillus/Intestinal microbiota/Probiotics/Pseudogobio vaillanti分类
农业科技引用本文复制引用
朱迪宁,吴偲,袁滨,林欢,朱秋强,郑俊贤,胡元庆,张丹凤..似鮈肠道菌群多样性及其潜在益生菌的筛选鉴定[J].水生生物学报,2024,48(12):2006-2017,12.基金项目
福建省自然科学基金(2020J01809、2021J05196和2021J01995)资助[Supported by the Natural Science Foundation of Fujian Province(2020J01809,2021J05196 and 2021J01995)] （2020J01809、2021J05196和2021J01995）
