水生生物学报2024，Vol.48Issue(12)：2081-2091,11.DOI:10.7541/2025.2024.0260
峡山水库水体和沉积物中微生物群落结构组成研究
COMPOSITION OF MICROBIAL COMMUNITY STRUCTURE IN WATER AND SEDIMENTS OF XIASHAN RESERVOIR IN SHANDONG PROVINCE
摘要
Abstract
Xiashan Reservoir,the premier reservoir in Shandong Province,serves as a crucial source of drinking water.However,escalating human activities and environmental pressures have posed formidable challenges to its ecological equilibrium.A notable gap in research regarding the microbial composition of its water body and sediments,which hold significant ecological and economic importance,has hindered our comprehension of its ecosystem functions and the development of effective conservation measures.To address this,we conducted a comprehensive analysis of microbial composition in June 2023 using high-throughput sequencing of bacterial 16S rRNA genes.Our findings revealed that the microbial diversity indices,including Sobs,Chao1,Ace,and diversity,were significantly higher in the sediments compared to the water body,underscoring the richer and more diverse microbial communities in the sediments.β-diver-sity analysis further emphasized distinct microbial compositions between these two environments.In the water body,Actinobacteria(57.65%),Proteobacteria(34.9%),and Bacteroidetes(6.22%)were the dominant bacterial phyla,with the most abundant genera being hgcI_clade(29.98%),Limnohabitans(17.06%),CL500-29_marine_group(11.97%),and Sporichthyaceae(7.12%).Conversely,the sediments were predominantly inhabited by Proteobacteria(39.63%),Bacteroidota(12.70%),Desulfobacterota(9.88%),Chloroflexi(6.79%),Nitrospirota(5.56%),and Acidobacteriota(3.52%),with Steroidobacteraceae(6.98%),Thermodesulfovibrionia(4.97%),Thiobacillus(4.33%),Sutterellaceae(4.07%),and SC-I-84(3.48%)as the leading genera.Although the dominant bacterial groups in Xiashan Reservoir exhibit similarities to those found in other domestic reservoirs,the presence of potential fish pathogens such as Flavobacterium and Aeromonas underscores the risk of fish diseases.Functional prediction analysis indicated that microbial functions in both water and sediments were largely congruent,with metabolic pathways,biosynthesis of secondary metabolites,microbial metabolism in diverse environments,biosynthesis of amino acids,and carbon metabolism being the most prominent metabolic pathways at level 3 prediction.Notably,immune diseases-related func-tions were significantly more abundant in the water body compared to the sediments.This study provides valuable insights into the ecological functions of the reservoir and offers essential background information for evaluating the effectiveness of future ecological management efforts.关键词
峡山水库/微生物多样性/高通量测序/细菌功能Key words
Xiashan Reservoir/Microbial diversity/High-throughput sequencing/Bacterial function分类
生物科学引用本文复制引用
刘振军,张倩倩,郭瑞莲,张艳,杨继成,吴振兵,李爱华..峡山水库水体和沉积物中微生物群落结构组成研究[J].水生生物学报,2024,48(12):2081-2091,11.基金项目
山东省潍坊市峡山水库管理局招标项目(SDZTXS-20211115) （SDZTXS-20211115）
国家自然科学基金(32073023)资助[Supported by the Bidding project of Shandong Weifang Xiashan Reservoir Authority(SDZTXS-20211115) （32073023）
the National Natural Science Foundation of China(32073023)] （32073023）
