水生生物学报2024，Vol.48Issue(12)：2109-2121,13.DOI:10.7541/2024.2024.0085
一株雷氏普罗维登斯菌的分离鉴定、生物学特性及其基因组学研究
ISOLATION,IDENTIFICATION,BIOLOGICAL CHARACTERISTICS,AND WHOLE-GENOME SEQUENCING OF A PROVIDENCIA RETTGERI STRAIN
摘要
Abstract
In order to identify the pathogen and its characteristics in diseased Cuora flavomarginata in Hangzhou,Zhejiang,in June 2020,a dominant strain HZ202006 was isolated from the liver of the diseased turtles.Morphological observations,16S rRNA sequence analysis,whole-genome sequencing,artificial infection experiments,and antimicro-bial sensitivity test were performed on this strain.The results confirmed that the strain is Providencia rettgeri,a short rod-shaped,Gram-negative bacterium.Whole-genome sequencing revealed that the complete genome sequence of this strain consists of a 4.8 Mbp double-stranded DNA and two plasmids.Annotation of the sequenced genes using the Comprehensive Antibiotic Resistance Database(CARD)and the Virulence Factors Database(VFDB)identified 20 resistance genes and four virulence factors in the bacterium.Antimicrobial sensitivity test demonstrated that HZ202006 was moderately sensitive only to amikacin,and resistant to 16 common antibiotics,including penicillin,cephalosporins,and aminoglycosides.This study isolated and identified a strain of P.rettgeri causing disease in C.flavomarginata,providing insights for disease control in aquaculture animals affected by this bacterium.关键词
雷氏普罗维登斯菌/分离/鉴定/药敏实验/全基因测序/黄缘闭壳龟Key words
Providencia rettgeri/Isolation/Identification/Antimicrobial susceptibility test/Whole genome sequenc-ing/Cuora flavomarginata分类
农业科技引用本文复制引用
廖鹏飞,李论,柳晨涛,丁钰杰,黄秋雅,胡祎玮,葛楚天,徐洁皓,章晓栋..一株雷氏普罗维登斯菌的分离鉴定、生物学特性及其基因组学研究[J].水生生物学报,2024,48(12):2109-2121,13.基金项目
浙江省教育厅一般科研项目(Y202353501) （Y202353501）
浙江省自然科学基金(LQ20C190007和LTGN23C190004) （LQ20C190007和LTGN23C190004）
浙江省水产新品种选育项目(2021C02069-8-3) （2021C02069-8-3）
宁波市重点技术研发项目(2021Z009)资助[Supported by General Scientific Research Project of Zhe-jiang Education Department(Y202353501) （2021Z009）
Zhejiang Provincial Natural Science Foundation of China(LQ20C190007,LTGN23C 190004) （LQ20C190007,LTGN23C 190004）
the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(2021C02069-8-3) （2021C02069-8-3）
Key Technology Research and Development Projects in Ningbo(2021Z009)] （2021Z009）
