水生生物学报2024，Vol.48Issue(12)：2122-2132,11.DOI:10.7541/2024.2024.0079
拮抗鲶爱德华氏菌的高地芽孢杆菌Yab4的鉴定及其应用前景
IDENTIFICATION AND APPLICATION PROSPECT OF BACILLUS ALTITUDI-NIS YAB4 ANTAGONIZING EDWARDSIELLA ICTALURI
摘要
Abstract
Thermostable strains isolated from intestine of catfish were screened for their antagonism activity against Edwardsiella ictaluri,the causative agent of enteric septicemia of catfish(ESC)and red head disease.Four ther-mostable strains were selected,and their antagonistic activity against E.ictaluri was compared.Adhesion to zebrafish larvae was determined as CFU/larva after exposure for 30min.The isolate Yab4 was found to be highly adhesive and was identified as Bacillus altitudinis based on a phylogenetic tree constructed using 16S rDNA and gyrB sequences.After 28d of feed supplementation with B.altitudinis Yab4,the growth performance of the treatment group is better than that of the control group,with a statistically significant difference observed between the two groups.Moreover,the innate immune indexes including alkaline phosphatase,lysozyme,and total proteins in serum increased significantly,and the expression levels of immune genes including IL-1β,TNF-α,and NF-κB p65 elevated significantly as well.Most importantly,the survival rate of the yellow catfish fed with B.altitudinis Yab4 supplementation reached 60%,marking a 35%increase compared to the control group when challenged with E.ictaluri HSN-1 infected intraperitoneally.This demonstrates that the potential of B.altitudinis Yab4 as a novel Bacillus probiotic to defend against E.ictaluri in catfish aquaculture through feed supplementation.关键词
高地芽孢杆菌/鲶爱德华氏菌/饲料添加剂/益生机理/黄颡鱼Key words
Bacillus altitudinis/Edwardsiella ictaluri/Feed supplement/Probiotic mechanism/Pelteobagrus fulvidraco分类
农业科技引用本文复制引用
陈长松,张钊,江修龙,韩冬,郑劲松,黄爱优,谢珍玉,谢海侠..拮抗鲶爱德华氏菌的高地芽孢杆菌Yab4的鉴定及其应用前景[J].水生生物学报,2024,48(12):2122-2132,11.基金项目
国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项(2023YFD2400501) （2023YFD2400501）
海南省热带水生生物技术重点实验室开放课题(SWJS202301)资助[Supported by the National Key Research and Development Program of China(2023YFD2400501) （SWJS202301）
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province(SWJS202301)] （SWJS202301）
