新疆农业科学2024，Vol.61Issue(10)：2396-2407,12.DOI:10.6048/j.issn.1001-4330.2024.10.007
基于防御酶与代谢组学分析贝莱斯芽孢杆菌JTB8-2诱导番茄拮抗瓜列当机制
Metabolomics analysis of Bacillus velezensis JTB8-2 induced tomato antagonism towards Orobanche aegyptiaca based on defense enzyme
摘要
Abstract
[Objective]Orobanche aegyptiaca is a completely parasitic,malignant weed.Bacillus velezen-sis JTB8-2 demonstrates potent inhibitory capabilities against O.aegyptiaca.Investigating its mechanism,so research into it might provide a solid theoretical foundation for the application of this strain in the field.[Methods]In order to investigate the molecular mechanism of B.elezensis JTB8-2-induced resistance to O.aegyptiaca in tomato,we employed pot culture,enzyme activity assays,and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MRM-MS/MS).[Results]The findings revealed that the application of B.elezensis JTB8-2 fermentation broth to tomatoes at 20,30,and 40 days after transplantation resulted in a parasitic O.aegyptiaca tubercle count of 0.33,0.17,and 0.33,respectively.This represented a reduction of 81.67%,96.96%,and 95.54%compared to the control group,and a reduction of 50.75%,79.52%,and 50.75%,respectively,compared to the NB medium treatment.The catalase(CAT)activity of tomato roots was found to be higher than that of both the medium treatment and water control group after 3,20,and 30 days.Moreover,differences were observed in the content of 11 metabolites among 28 plant hor-mone metabolites in tomato roots.The levels of cytokinins and auxin compounds in tomato roots treated with strain JTB8-2 were higher than those in the water control group.[Conclusion]The upregulation of catalase activity,cytokinin,and auxin-related hormones in tomato roots treated with strain JTB8-2 may elicit a sys-temic resistance response in the plant,thereby reducing the severity of O.aegyptiaca parasitism.关键词
瓜列当/贝莱斯芽孢杆菌/防御酶/代谢组学/诱导抗性Key words
Orobanche aegyptiaca/Bacillus velezensis/defensive enzyme/metabolomics/induced resist-ance分类
农业科技引用本文复制引用
朱夏芬,何伟,罗文芳,周军辉,李克梅,许建军..基于防御酶与代谢组学分析贝莱斯芽孢杆菌JTB8-2诱导番茄拮抗瓜列当机制[J].新疆农业科学,2024,61(10):2396-2407,12.基金项目
新疆维吾尔自治区自然科学基金项目(2022D01A91) （2022D01A91）
农业科技创新稳定支持项目(xjnkywdzc-2022004) （xjnkywdzc-2022004）
新疆现代农业产业技术体系(XJARS-07) Project of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01A91) （XJARS-07）
Project for Supporting Stable Agricul-tural Sci-Tech Renovation(xjnkywdzc-2022004) （xjnkywdzc-2022004）
Earmarked Fund for XJARS(XJARS-07) （XJARS-07）
