畜牧兽医杂志2024，Vol.43Issue(6)：29-33,5.DOI:10.7606/j.issn.1004-6704.2024.06.008
滩羊卵母细胞体外成熟和孤雌激活试验
In Vitro Maturation and Parthenogenetic Activation Test of Tan Sheep Oocytes
杨文琳 1许斌 1李冉 2张秀陶 1李生虎1
作者信息
- 1. 宁夏职业技术学院,宁夏银川 750021
- 2. 西北农林科技大学动物科技学院,陕西杨凌 712100
- 折叠
摘要
Abstract
To establish an in vitro maturation and parthenogenetic activation culture system for Tan sheep oocytes,Tan sheep oocytes derived from ex vivo ovaries were subjected to in vitro maturation culture and parthenogenetic activation was per-formed using chemical activation method.The results showed that the average number of eggs obtained from detached ovaries of Tan sheep was 3.07,the maturation rate of oocytes was 54.78％,the cleavage rate was 61.92％,and the blastocyst rate was 17.72％.There was no significant difference between the replicates of the experiment(P＞0.05).In summary,the culture sys-tem established in this experiment is relatively stable and suitable for the in vitro maturation culture of Tan sheep oocytes,lay-ing a laboratory foundation for the production of in vitro fertilized embryos and cloned embryos.关键词
滩羊/卵母细胞/体外成熟培养/产业发展Key words
Tan sheep/oocytes/in vitro maturation culture/industrial development分类
农业科技引用本文复制引用
杨文琳,许斌,李冉,张秀陶,李生虎..滩羊卵母细胞体外成熟和孤雌激活试验[J].畜牧兽医杂志,2024,43(6):29-33,5.
