畜牧兽医杂志2024，Vol.43Issue(6)：58-62,5.DOI:10.7606/j.issn.1004-6704.2024.06.015
复方中药制剂对猪传染性胃肠炎病毒的体外作用研究
Study on the Effect of Compound Chinese Medicine Preparation on Porcine Transmissible Gastroenteritis Virus in Vitro
摘要
Abstract
In order to investigate the inhibitory effect of traditional Chinese medicine compound preparation on the prolifera-tion of porcine transmissible gastroenteritis virus(TGEV)on PK-15 cells in vitro.In the experiment,four formulas of com-pound Chinese medicine preparations were selected,which were composed of seven traditional Chinese medicine extracts of Pul-satilla chinensis,Phellodendron chinense,Green husk,fried Atractylodes,jiao Sanxian,Herba agrimoniae,woody fragrance.Three modes of action were adopted:adding drugs first and then adding viruses,adding drugs and viruses after a period of time,adding viruses first and then adding drugs.The inhibitory adsorption and penetration of drugs on viruses,direct killing and inhi-bition of replication were observed.The results showed that the compound Chinese medicine preparation at a dose of 1 mg/mL had an effect on the proliferation of TGEV on PK-15 cells in vitro.The formula 2 was superior to the other three formulas,which had a good inhibitory effect on TGEV,and the direct killing effect had the best protective effect on cells.The cell survival rate was 75％(P＜0.01),and an effective compound Chinese medicine preparation with three ways of anti-virus was screened.The results can provide a scientific basis for the subsequent screening of anti-TGEV drugs.关键词
复方中药制剂/猪传染性胃肠炎病毒/体外作用Key words
compound Chinese medicine preparation/porcine transmissible gastroenteritis virus/action in vitro分类
农业科技引用本文复制引用
李元新,容维中,成伟伟,杨明,赵子惠,周瑶,王佳..复方中药制剂对猪传染性胃肠炎病毒的体外作用研究[J].畜牧兽医杂志,2024,43(6):58-62,5.基金项目
甘肃省农业生物技术研究与应用开发项目(GNSW-2011-24) （GNSW-2011-24）
甘肃省青年科技基金计划(21JR7RA718) （21JR7RA718）
