间歇性禁食减轻内毒素诱导小鼠葡萄膜炎的研究OA

Objective:To investigate the protective effects of intermittent fasting(IF)on endotoxin-induced uveitis in mice and its potential anti-inflammatory mechanisms.Methods:Mice were randomly divided into three groups:control(Ctr)group,endotoxin-induced uveitis(EIU)group,and IF+EIU group.The IF regimen followed a 16:8 fasting scheme(feeding from 9:00 to 17:00).The control group received intravitreal injections of PBS,while the other two groups received intravitreal injections of lipopolysaccharide(LPS).After modeling,fasting blood glucose and body weight of the mice were monitored.Inflammation levels were assessed using OCT and H&E staining.Retinal flat mounts were used for evaluating neuroinflammation.BV2 cells were divided into Ctr group,LPS group,and starvation(LG)+LPS group.The expression levels of related proteins and mRNA were detected using WB and RT-qPCR.Results:IF had no significant effect on body weight but caused a significant decrease in blood glucose,which gradually recovered.From the middle stage of the disease,mice in the IF intervention group show edretinal edema recovery,significantly reduced intravitreal inflammatory exudation and cell infiltration compared to the EIU group(P<0.01).IF reversed LPS-induced microglial activation and significantly alleviated damage to retinal ganglion cells and nerve fibers(P<0.05).Starvation culture significantly inhibited LPS-induced expression levels of p-STAT1/3 and iNOS proteins in BV2 cells(P<0.05)and significantly reduced the expression levels of inflammatory factors such as iNOS and IL-6.Conclusion:IF can accelerate the resolution of EIU inflammation,reduce inflammatory damage to tissue structures,and inhibit pro-inflammatory activation of microglia.