NRF2激动剂衣康酸4-辛酯对猪伪狂犬病病毒的抑制作用研究OA北大核心CSTPCD

To identify small molecules that can effectively inhibit porcine pseudorabies virus(PRV),we performed a high-throughput screening of a small molecule library by flow cytometry analysis technology and evaluated the effect of identified small molecules on PK15 cell viability by CCK-8 as-say.The inhibitory effects of small molecules on PRV were investigated by detecting PRV gB protein ex-pression and determining viral titer by TCID50.The specific stages of the PRV life cycle that are hin-dered by small molecules were performed by Western-blot assays.The results showed that the three small molecules with the most obvious inhibitory effects on PRV were identified by flow cytometry analysis.Cyanosafracin B and Staurosporine showed high cytotoxicity,while 4-Octl itaconate(4-OI)is non-toxic even at a concentration of 300 μmol/L.4-OI showed strong antiviral activity upon PRV infection of dif-ferent MOIs,as evidenced by a dose-dependent decrease in PRV progeny virus titer and PRV gB protein ex-pression.4-OI and its target protein NRF2 can inhibit the replication of PRV in cells without affecting the adsorption of PRV to PK15 cells and its internalization process.This study shows that 4-OI is a po-tentially efficient small-molecule drug that inhibits PRV,providing a new reference for PRV preven-tion and control.