中国兽医科学2024，Vol.54Issue(11)：1421-1429,9.DOI:10.16656/j.issn.1673-4696.2024.0182
NRF2激动剂衣康酸4-辛酯对猪伪狂犬病病毒的抑制作用研究
NRF2 agonist 4-octyl itaconate inhibits the proliferation of porcine pseudorabies virus
摘要
Abstract
To identify small molecules that can effectively inhibit porcine pseudorabies virus(PRV),we performed a high-throughput screening of a small molecule library by flow cytometry analysis technology and evaluated the effect of identified small molecules on PK15 cell viability by CCK-8 as-say.The inhibitory effects of small molecules on PRV were investigated by detecting PRV gB protein ex-pression and determining viral titer by TCID50.The specific stages of the PRV life cycle that are hin-dered by small molecules were performed by Western-blot assays.The results showed that the three small molecules with the most obvious inhibitory effects on PRV were identified by flow cytometry analysis.Cyanosafracin B and Staurosporine showed high cytotoxicity,while 4-Octl itaconate(4-OI)is non-toxic even at a concentration of 300 μmol/L.4-OI showed strong antiviral activity upon PRV infection of dif-ferent MOIs,as evidenced by a dose-dependent decrease in PRV progeny virus titer and PRV gB protein ex-pression.4-OI and its target protein NRF2 can inhibit the replication of PRV in cells without affecting the adsorption of PRV to PK15 cells and its internalization process.This study shows that 4-OI is a po-tentially efficient small-molecule drug that inhibits PRV,providing a new reference for PRV preven-tion and control.关键词
药物筛选/衣康酸4-辛酯(4-OI)/猪伪狂犬病病毒(PRV)/抗病毒作用Key words
drug screening/4-octylitaconic acid(4-OI)/porcine pseudorabies virus(PRV)/antivi-ral effects分类
农业科技引用本文复制引用
方春艳,杨发誉,朱紫祥,郑海学,包世俊,魏衍全,张石磊..NRF2激动剂衣康酸4-辛酯对猪伪狂犬病病毒的抑制作用研究[J].中国兽医科学,2024,54(11):1421-1429,9.基金项目
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
甘肃省基础研究创新群体项目(23JRRA561) （23JRRA561）
