基于大肠杆菌表达系统高效生产外源蛋白的自诱导培养基的研发OA北大核心CSTPCD

The self-induction medium suitable for the expression system of Escherichia coli to ef-ficiently express recombinant protein was developed to increase the production of antigens,reduce costs and increase efficiency for enterprises.This study will be developed self-induction medium suitable for Escherichia coli expression system to express recombinant protein efficiently.The recom-binant expression plasmid with the gene of multi-epitope for foot-and-mouth disease antigen developed previously in our laboratory was used to verification of self-inducing medium.An optimal self-induc-ing culture medium was developed through orthogonal experiment and suitable induction temperature.The results showed that the yield of foot-and-mouth disease antigen produced by highly efficient self-in-duced medium was 6 times that of ordinary LB medium,and the cost of producing 1 mg foot-and-mouth dis-ease antigen was reduced by 78％.This indicated that we have successfully developed a self-induction medium suitable for prokaryotic expression systems.The good cost-effectiveness will help biological products enterprises reduce costs,improve product competitiveness,and create products with broad ap-plication prospects.