中国兽医科学2024，Vol.54Issue(11)：1430-1436,7.DOI:10.16656/j.issn.1673-4696.2024.0177
基于大肠杆菌表达系统高效生产外源蛋白的自诱导培养基的研发
Development of self-induction medium for efficient production of recombinant protein based on Escherichia coli expression system
摘要
Abstract
The self-induction medium suitable for the expression system of Escherichia coli to ef-ficiently express recombinant protein was developed to increase the production of antigens,reduce costs and increase efficiency for enterprises.This study will be developed self-induction medium suitable for Escherichia coli expression system to express recombinant protein efficiently.The recom-binant expression plasmid with the gene of multi-epitope for foot-and-mouth disease antigen developed previously in our laboratory was used to verification of self-inducing medium.An optimal self-induc-ing culture medium was developed through orthogonal experiment and suitable induction temperature.The results showed that the yield of foot-and-mouth disease antigen produced by highly efficient self-in-duced medium was 6 times that of ordinary LB medium,and the cost of producing 1 mg foot-and-mouth dis-ease antigen was reduced by 78％.This indicated that we have successfully developed a self-induction medium suitable for prokaryotic expression systems.The good cost-effectiveness will help biological products enterprises reduce costs,improve product competitiveness,and create products with broad ap-plication prospects.关键词
大肠杆菌/表达/自诱导培养基/乳糖Key words
Escherichia coli/expression/self-induction medium/lactose分类
农业科技引用本文复制引用
侯茁,邵军军,刘伟,高闪电,彭德财,宁小雨,马云云,温升辉,常惠芸..基于大肠杆菌表达系统高效生产外源蛋白的自诱导培养基的研发[J].中国兽医科学,2024,54(11):1430-1436,7.基金项目
兰州市科技项目(2021-1-12) （2021-1-12）
甘肃省青年科技基金项目(21JR7RA025) （21JR7RA025）
