中国兽医科学2024，Vol.54Issue(11)：1443-1450,8.DOI:10.16656/j.issn.1673-4696.2024.0180
非洲猪瘟病毒p30和pK205R双抗原间接ELISA检测方法的建立
Establishment of indirect ELISA based on p30 and pK205R dual-proteins for detection of African swine fever virus
摘要
Abstract
In order to develop a reliable and rapid serological detection method,a dual-antigens indirect ELISA was established with structural protein p30 and non-structural protein pK205R of African swine fever virus(ASFV)as diagnostic antigens for the detection of ASFV antibodies.The results showed that the method had no cross-reactivity with positive sera of common swine-origin pathogens,such as pseudorabies virus,classical swine fever virus,Mycoplasma hyopneumoniae,porcine circovirus and porcine reproductive and respiratory syndrome virus.The sensitivity to ASF positive serum reached 1∶12 800.The coefficient of variation of intra-batch was 7.95％and the coefficient of variation of inter-batch repeatability was 9.18％,both less than 10％.Total 130 clinical pig serum samples were tested by the method and a ID.vet commercial ELISA kit,the positive conformity rate reached 92.19％,the negative conformity rate reached 86.36％,and the total conformity rate reached 89.23％.In summary,this study successfully established an ASFV p30 and pK205R dual-antigens indirect ELISA,which provided a new technical support for the detection of ASFV-specific antibodies in pig serum.关键词
非洲猪瘟病毒/p30蛋白/pK205R蛋白/间接ELISAKey words
African swine fever virus/p30/pK205R/indirect ELISA分类
农业科技引用本文复制引用
何佳依,贾红,郑龙三,吴晓东,冯志新,钱莺娟,邹艳丽,王燕,刘珊,苗雨润,任炜杰,王振忠,白昀,陈欢..非洲猪瘟病毒p30和pK205R双抗原间接ELISA检测方法的建立[J].中国兽医科学,2024,54(11):1443-1450,8.基金项目
"十四五"国家重点研发计划项目(2021YFD1801200,2022YFD1800500) （2021YFD1801200,2022YFD1800500）
国家重点研发计划政府间国际科技创新合作重点专项(2019YFE0107300) （2019YFE0107300）
江苏省重点研发计划(现代农业)重点项目(SBE2020310346) （现代农业）
