中国兽医科学2024，Vol.54Issue(11)：1491-1501,11.DOI:10.16656/j.issn.1673-4696.2024.0175
PRRSV阳性猪源肺炎克雷伯菌的分离鉴定及特性分析
Isolation,identification and characterization of Klebsiella pneumoniae secondary to PRRSV infection
摘要
Abstract
The aim of this study was to investigate the bacterial pathogens and their biological characteristics of secondary infection after clinical infection with porcine reproductive and respi-ratory syndrome virus(PRRSV),and to provide a research basis for the effective prevention and control of PRRSV secondary bacterial infection.The pleural effusion of dead piglets infected with PRRSV was asep-tically collected for bacterial isolation and purification.The isolated bacteria were subjected to morphological identification,molecular biological identification,drug sensitivity test,drug resis-tance gene and virulence gene detection,and the pathogenicity of the isolated bacteria was further evaluated by animal experiments.In result,the strain was gram-negative short bacilli,and the biochem-ical tests of glucose,urea,peptone water,citrate and lysine were positive,and it was preliminarily identified as Klebsiella bacteria.The sequencing results of PCR amplification products of bacterial universal primers showed that the homology between the bacteria and Klebsiella pneumoniae from broiler chickens in Pakistan was 99.77％,and the isolated strain was further identified as Klebsiella pneumo-niae.The results of drug sensitivity test showed that the strain of Klebsiella pneumoniae had strong resistance to tetracycline,erythromycin,azithromycin,penicillin,ampicillin and lincomycin,and was not sensitive to streptomycin and kanamycin.The bacteria carried 3 drug resistance genes and 6 viru-lence genes,and had strong pathogenicity to BALB/c mice.The mortality rate was 100％at 7.0× 108CFU/mL challenge.In conclusion,in this study,a strain of Klebsiella pneumoniae was isolated from dead piglets infected with PRRSV,which had multiple drug resistance and strong pathogenicity.This study shows that Klebsiella pneumoniae is an important secondary pathogen of PRRSV infection,which has im-portant guiding significance for the prevention and treatment of secondary bacterial infection in PRRSV positive pigs.关键词
猪繁殖与呼吸综合征病毒/肺炎克雷伯菌/毒力/耐药性/致病性Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/Klebsiella pneumoniae/viru-lence/drug resistance/pathogenicity分类
农业科技引用本文复制引用
常辉,郑海学,齐萌,王玉淼,李静,李洋,荆扬,郑紫方,裴秀秀,陈劭,肖书奇..PRRSV阳性猪源肺炎克雷伯菌的分离鉴定及特性分析[J].中国兽医科学,2024,54(11):1491-1501,11.基金项目
国家重点研发计划项目(2022YFD1800304-06) （2022YFD1800304-06）
甘肃省联合科研基金重大项目(23JRRA1476) （23JRRA1476）
甘肃省自然科学基金项目(23JRRA1153) （23JRRA1153）
