中国免疫学杂志2024，Vol.40Issue(12)：2537-2542,6.DOI:10.3969/j.issn.1000-484X.2024.12.013
胡椒碱调节AC/PKA/CREB信号通路对脑梗死大鼠海马神经元凋亡的影响
Effect of piperine on AC/PKA/CREB signaling pathway on hippocampal neuron apoptosis in rats with cerebral infarction
摘要
Abstract
Objective:To investigate the effect and of piperine(PIP)on nerve injury in rats with cerebral infarction.Meth-ods:The rat model of cerebral infarction was prepared by modified thread embolism method.The rats were divided into Sham group,Model group,piperine group(PIP group,20 mg/kg piperine),piperine+PKA inhibitor group(PIP+H-89 group,20 mg/kg PIP+5 mg/kg H-89).The rats were scored for neurological impairment,the volume of cerebral infarction,neuronal damage and neuronal apoptosis were observed,and the levels of cAMP,IL-1β and IL-6 in hippocampus and the protein expressions of GFAP,NSE,AC6,PKA,p-CREB,CREB and BDNF were detected.Results:Compared with Sham group,Model group had large cerebral infarction,cerebral in-farction volume,the neurological deficit score,neuronal apoptosis rate,the levels of IL-6 and IL-1β in hippocampus,and number of GFAP positive cells,the positive expression rates of NSE protein increased,the number of Nissl bodies,and the expressions of cAMP,AC6,PKA,p-CREB and BDNF proteins in hippocampus decreased(P<0.05);compared with Model group,cerebral infarc-tion volume,the neurological deficit score,neuronal apoptosis rate,the levels of IL-6 and IL-1β in hippocampus,and number of GFAP positive cells,the positive expression rates of NSE protein in the PIP group decreased,the number of Nissl bodies,and the ex-pressions of cAMP,AC6,PKA,p-CREB and BDNF proteins in hippocampus increased(P<0.05);the effect of piperine in improving nerve injury in rats with cerebral infarction was able to be reversed by H-89.Conclusion:PIP can inhibit the apoptosis of hippocampal neurons,and improve the neural injury induced by cerebral infarction by activating AC/PKA/CREB signal pathway.关键词
胡椒碱/脑梗死/AC/PKA/CREB信号通路/神经元Key words
Piperine/Cerebral infarction/AC/PKA/CREB signaling pathway/Neuron分类
医药卫生引用本文复制引用
李德炎,黄宗文,吴亚丹,陈秀红..胡椒碱调节AC/PKA/CREB信号通路对脑梗死大鼠海马神经元凋亡的影响[J].中国免疫学杂志,2024,40(12):2537-2542,6.基金项目
2020年海南省自然科学基金面上项目(820RC778). （820RC778）
