中国免疫学杂志2024，Vol.40Issue(12)：2598-2603,6.DOI:10.3969/j.issn.1000-484X.2024.12.023
WDR4基因在肺腺癌中的表达、预后及免疫特征分析
Expression,prognosis and immune characteristics of WDR4 gene in lung adenocarcinoma
摘要
Abstract
Objective:To investigate clinical significance of WDR4 gene expression,prognosis and immune infiltration in lung adenocarcinoma(LUAD).Methods:Clinicopathological data and WDR4 mRNA expression data of 535 patients with LUAD were collected through TCGA database,WDR4 protein expression data of 111 patients with LUAD were collected through CPTAC database.Associations between WDR4 expression and various clinicopathological features,as well as with overall survival in LUAD were ana-lyzed by R software.Diagnostic value of WDR4 expression in LUAD were assessed by ROC.Correlation between WDR4 expression and immune infiltration in tumor microenvironment in LUAD were investigated by Estimates and ssGSEA algorithms.Results:WDR4 mRNA expression in LUAD was significantly higher than that in normal lung tissues(all P<0.001).Protein expression of WDR4 in LUAD was also significantly up-regulated(P<0.05).Univariate analysis showed that expression of WDR4 was correlated with lymph node metastasis(P<0.05).Log-rank test showed that overall survival of patients with LUAD in WDR4 high expression group was significantly lower than that in low expression group(P=0.004).Multivariate Cox analysis revealed that T stage,lymph node metasta-sis and WDR4 mRNA expression remained independently associated with prognosis of LUAD patients.Death risk in patients with high expression of WDR4 mRNA was 1.478 times that of patients with low expression(95%CI:1.041～2.097).ROC curve analysis showed that WDR4 mRNA expression predicted diagnosis of LUAD to high extent(AUC=0.912,95%CI:0.885～0.935).High expression of WDR4 was negatively correlated with tumor microenvironment immune infiltration(all P<0.05).Conclusion:WDR4 expression in LUAD is significantly higher than that in normal lung tissue,and high WDR4 expression indicates poor prognosis in patients with LUAD.High expression of WDR4 inhibits immune infiltration in tumor microenvironment.WDR4 may be a novel potential biomarker for diagnosis and prognosis of patients with LUAD and therapeutic target.关键词
WDR4/肺腺癌/生物信息学/预后/免疫浸润Key words
WDR4/Lung adenocarcinoma/Bioinformatic/Prognosis/Immune infiltration分类
医药卫生引用本文复制引用
高军,林欣,宋一祎,朱珍,李虹..WDR4基因在肺腺癌中的表达、预后及免疫特征分析[J].中国免疫学杂志,2024,40(12):2598-2603,6.基金项目
国家自然科学基金青年科学基金项目(82004432). （82004432）
