中国兽医科学2024，Vol.54Issue(11)：1554-1559,6.DOI:10.16656/j.issn.1673-4696.2024.0191
褪黑素体内缓解急性铅中毒小鼠卵母细胞体外成熟的研究
Melatonin alleviates in vitro maturation of mice oocytes with acute lead poisoning
摘要
Abstract
In order to investigate the effect of in vivo injection of melatonin on the maturation of oocytes in mice with acute lead poisoning,the oocytes of ICR mice aged 6-8 weeks with similar body con-dition were intraperitoneally injected with different doses of melatonin(20,30,40 mg/kg weight)in vivo,and the release of the first polar body was used as a sign of oocyte maturation.The optimal injection concentration of melatonin was determined,and the impact of melatonin injection on the in vitro matu-ration of mice oocytes with acute lead poisoning was analyzed by assessing reactive oxygen species,ATP content,mitochondrial distribution and apoptosis.The results showed that intraperitoneal injection of 40 mg/kg melatonin significantly reduced the reactive oxygen species in oocytes,the abnormal dis-tribution ratio of mitochondria,and the early apoptosis rate of oocytes.Additionally,it and signifi-cantly increased the first polar body excretion rate and ATP content of oocytes.In conclusion,in-traperitoneal injection of 40 mg/kg melatonin enhances the effect of oocyte maturation in vitro in mice with acute lead poisoning.关键词
卵母细胞/体外成熟/褪黑素/急性铅中毒/小鼠Key words
oocyte/in vitro maturation/melatonin/acute lead poisoning/mice分类
农业科技引用本文复制引用
张天颖,牛浩原,瞿静雯,李拥军..褪黑素体内缓解急性铅中毒小鼠卵母细胞体外成熟的研究[J].中国兽医科学,2024,54(11):1554-1559,6.基金项目
江苏现代农业产业技术体系建设项目[JATS(2023)448] （2023）
江苏省卓越博士后计划项目 （）
