局解手术学杂志2024，Vol.33Issue(12)：1039-1043,5.DOI:10.11659/jjssx.03E024080
二氢杨梅素对子宫内膜癌细胞肿瘤生物学特性的影响机制研究
Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells
摘要
Abstract
Objective To explore the effects of dihydromyricetin on the proliferation,apoptosis,and invasion of endometrial cancer(EC)cells and its possible mechanisms.Methods Ishikawa cells in the logarithmic growth phase were taken and divided into the control group,20 µmol/L dihydromyricetin intervention group,40 µmol/L dihydromyricetin intervention group,and 80 µmol/L dihydromyricetin intervention group,which were treated with different final concentrations of dihydromyricetin(0 µmol/L,20 µmol/L,40 µmol/L,80 µmol/L).Then,CCK-8 assay and flow cytometry were used to detect the effects of dihydromyricetin on the cell proliferation and apoptosis.Transwell experiment was used to detect the effect of dihydromyricetin on the cell invasion.qRT-PCR and Western blot were used to detect the effects of dihydromyricetin on the cell expression of miR-21 and PTEN.Results Compared with the control group,the cell proliferation inhibition rate and apoptosis rate in the dihydromyricetin intervention group were significantly increased(P<0.05),and gradually increased with the increase of dihydromyricetin concentration(P<0.05).Compared with the control group,the numbers of migration and invasion cells in the dihydromyricetin intervention group were significantly decreased(P<0.05),and gradually decreased with the increase of dihydromyricetin concentration(P<0.05).Compared with the control group,the cell expression of miR-21 in the dihydromyricetin intervention group was significantly decreased(P<0.05),and gradually decreased with the increase of dihydromyricetin concentration(P<0.05),the expression levels of PTEN mRNA and protein were significantly increased(P<0.05),and gradually increased with the increase of dihydromyricetin concentration(P<0.05).Conclusion Dihydromyricetin can inhibit the growth and metastasis of EC cells,and the inhibitory effect is positively correlated with its concentration.The mechanism may be related to the effect of dihydromyricetin on the miR-21/PTEN signaling pathway of EC cells.关键词
二氢杨梅素/子宫内膜癌/细胞增殖/细胞凋亡/细胞侵袭/miR-21/PTENKey words
dihydromyricetin/endometrial cancer/cell proliferation/cell apoptosis/cell invasion/miR-21/PTEN分类
医药卫生引用本文复制引用
崔伟华,宋静莹,乔淑新,段书众..二氢杨梅素对子宫内膜癌细胞肿瘤生物学特性的影响机制研究[J].局解手术学杂志,2024,33(12):1039-1043,5.基金项目
河北省医学科学研究重点课题计划项目(20220413) （20220413）
