赣南医科大学学报2024，Vol.44Issue(11)：1100-1104,5.DOI:10.3969/j.issn.1001-5779.2024.11.002
橙皮苷在兔眼青光眼滤过术后抑制滤过泡瘢痕化的作用研究
Effect of hesperidin on inhibiting scarring after glaucoma filtration surgery in rabbits
摘要
Abstract
Objective:To investigate the inhibitory effect of hesperidin on follicular scarring after glaucoma filtration surgery in rabbit eyes.Methods:Fifty-four healthy adult New Zealand white rabbits with 108 eyes(male)were selected to construct a glaucoma filtration surgery model.Rabbits were randomly divided into hesperidin group,mitomycin C group and normal saline group,with 18 rabbits in each group.In each group,1.92 mg·mL-1 hesperidin cotton tablets,0.2 mg·mL-1 mitomycin C cotton tablets and normal saline cotton tablets were placed under fascial sac and scleral flap,respectively.The cotton tablets were removed 3 minutes later and fully washed with normal saline.The morphology of anterior segment and filtering bleb were observed and the changes of IOP were monitored.At 7,14 and 28 days after operation,6 rabbits were killed randomly in each group,and 12 eyeballs were taken for pathological examination to observe the scar hyperplasia of the filtration channels.Results:Intraocular pressure significantly decreased in the hesperidin group and mitomycin C group compared with the saline group at all time points after filtration surgery,the differences were statistically significant(P＜0.05).Compared with the control group,the hesperidin group and mitomycin C group exhibited stronger filtration and more diffuse and elevated filtering blebs.Over time,the filtering bleb gradually decreased and became flatter in all groups.This trend was more obvious in the saline group.Pathological sections of the filtration port showed consistently open filtration channels in the hesperidin group and mitomycin C group at 7,14,and 28 days after surgery,and a few inflammatory cells and fibroblasts were observed.However,the filtration channels in the saline group gradually closed,and substantial inflammatory cell infiltration and fibroblast hyperplasia were observed.Conclusion:Hesperidin may be an effective anti-scarring drug that provides good surgical results in experimental rabbit glaucoma filtration surgery.关键词
橙皮苷/青光眼/小梁切除术/瘢痕化/兔Key words
Hesperidin/Glaucoma/Filtration surgery/Scarring/Rabbit分类
医药卫生引用本文复制引用
刘芳,余星慧,曾令根,谢连凤,谭述香,刘晶,刘琳琳..橙皮苷在兔眼青光眼滤过术后抑制滤过泡瘢痕化的作用研究[J].赣南医科大学学报,2024,44(11):1100-1104,5.基金项目
江西省教育厅科学技术研究项目(GJJ190796) （GJJ190796）
