华东师范大学学报（自然科学版）Issue(6)：74-85,12.DOI:10.3969/j.issn.1000-5641.2024.06.007
典型场地土壤剖面中抗生素及其抗性基因的分布特征
Characterization of the distribution of antibiotics and their resistance genes in soil profiles of a typical site
摘要
Abstract
The presence of a variety of environmental contaminants in the soils of steelworks seriously threatens the safety of urban soil and groundwater.Emerging pollutants in deep soils,such as antibiotics and antibiotic resistance genes(ARGs),have not been studied extensively and require timely monitoring and treatment.In this study,the Mingfang Iron and Steel Plant in Handan,Hebei Province,was selected as the research area,and three 4-meter soil columns were collected.The contents of 17 antibiotics in four categories(sulfonamides,fluoroquinolones,macrolides,and tetracyclines)were detected by ultra-performance liquid chromatography-tandem mass spectrometry.The types and abundances of ARGs in the soil at different depths were investigated by metagenomic sequencing.The results showed that the total antibiotic concentrations ranged from 2 593.2 to 4 279.5 ng/g,among which sulfonamide antibiotics(SAs)were 166.1 to 1 103.8 ng/g,fluoroquinolone antibiotics(FQs)were 993.3 to 1330.4 ng/g,macrolide antibiotics(MLs)ranged from 4.0 to 104.8 ng/g,and tetracycline antibiotics(TCs)ranged from 1 269.5 to 2 077.6 ng/g,with mild to moderate levels of contamination.Soil profile analysis demonstrated that the percentage of SAs were higher in the topsoils(0-150 cm)than in the deep soils(150-400 cm).Contrastingly,MLs accumulated more in deep soils(350-400 cm).The percentages of TCs and FQs were higher in all soil layers and they were the main antibiotic classes detected.The abundance of ARGs corresponding to the four classes of antibiotics decreased with increasing soil depth.The relative abundance of macrolide ARGs was the highest,with the read number of the detected sequences ranging from 0.92 × 105 to 1.44 X 105.The statistical chart of species contributions indicated that Actinobacteria made the greatest contribution to the abundance of ARGs in all four major categories.In conclusion,this study revealed the distribution and migration characteristics of antibiotics in the site soils.Deep soil pollution by ARGs is a matter of concern as it may lead to groundwater contamination and the increased distribution of antibiotic-resistant bacteria.关键词
钢铁冶炼厂场地/土壤柱/抗生素/抗生素抗性基因/宏基因组测序Key words
steelworks site/soil column/antibiotics/antibiotic resistance genes/metagenomics sequencing分类
资源环境引用本文复制引用
关铭茵,刘欣然,刘敏,杨静,谢泽莹,张庆玲,李倩..典型场地土壤剖面中抗生素及其抗性基因的分布特征[J].华东师范大学学报（自然科学版）,2024,(6):74-85,12.基金项目
国家重点研发计划(2020YFC1806700) （2020YFC1806700）
国家自然科学基金(42230505,41730646,42071134) （42230505,41730646,42071134）
