黑龙江农业科学Issue(12)：12-20,9.DOI:10.11942/j.issn1002-2767.2024.12.0012
农家豌豆品种白粉病抗性基因分子标记筛查
Screening of Powdery Mildew Resistance Genes in Farm Pea Varieties by Molecular Markers
摘要
Abstract
In order to study the resistance to powdery mildew of farm pea and obtain disease-resistant varieties,7 markers linked to 4 resistance genes of 29 farm pea varieties were screened by molecular marker technology and their resistance to powdery mildew of pea was predicted.The results showed that target bands of 650,1 600,1 400,637,880,1 200 and 874 bp were obtained by PCR amplification of 7 molecular markers linked to powdery mildew resistance genes in pea.The distribution frequency of the seven markers was high,ranging from 65％to 90％,but the distribution frequency was different.Among the 29 pea materials tested,only mountain peas from Qingshuihe did not contain the linked markers of pea mildew resistance gene,while the other 28 varieties contained 1-7 markers,among which the resistance gene er1 was detected only in grey-green wild peas from Helin and arrow end peas from Zhangye Minle.And the pink wild peas from Helin contained only the resistance gene Er3,three-pronged peas from Fengzhen contained only the resistance genes er2 and Er3.And three-pronged peas from Liangcheng contained three resistance genes er1,er2,and Er3 at the same time,and without the susceptibility gene Er1.The remaining 23 materials all contain both resistance genes er1,er2,Er3 and disease susceptibility gene Er1.The molecular markers contained in pea varieties of different origin had certain regional characteristics,and the molecular markers contained in different varieties had certain differences.The molecular markers of resistance of the same name varieties from different origin were diverse,and the resistance markers of the same name varieties from the same region were the same.关键词
豌豆/白粉病/抗性基因/分子标记/筛查Key words
pea/powdery mildew/resistance gene/molecular marker/screening引用本文复制引用
赵永秀,张凌硕,宁琳懿睿,秦馨悦,刘倬宇,牛素清..农家豌豆品种白粉病抗性基因分子标记筛查[J].黑龙江农业科学,2024,(12):12-20,9.基金项目
内蒙古科技计划项目(2020GG0052) （2020GG0052）
2023年度内蒙古第十二批"草原英才"个人项目(牛素清) （牛素清）
内蒙古大学本科毕业论文设计经费项目. （）
