棉花立枯病拮抗真菌的筛选鉴定及防病作用研究OA

In order to screen beneficial microorganisms for effectively controlling cotton blight,dual culture method was used to screening the highest biocontrol effect to the pathogen of cotton blight,the strain was identified based on the analysis of morphological traits and rDNA internal transcribed spacers(ITS),clarify whether this strain has a heavy parasitic effect on Rhizoctonia solani,and its biocontrol effect on cotton blight was clarified through pot experiments.The results showed that one strain HBHD-1 with high inhibitory effect on the pathogen of cotton blight was screened out from 24 strains of Trichoderma,with an inhibition rate of 89％.Based on morphological and ITS sequence analysis methods,the strain was identified as Trichoderma asperellum,which can produce heavy parasitic effects on Rhizoctonia solani.The results of the pot experiment showed that the biocontrol effect of inoculating Rhizoctonia solani and Trichoderma simultaneously on cotton could reach 70.85％.